製品の概要

  • 製品名
    Anti-Kinesin 5 A + B + C antibody
    Kinesin 5 A + B + C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Kinesin 5 A + B + C
  • アプリケーション
    適用あり: ICC/IF, IP, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Raised against a 20 amino acid peptide from near the carboxy terminus of human kinesin 5 (GenBank accession no. Q12840).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500.
IP 1/400. (see Abreview)
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 110 kDa).
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.
  • 細胞内局在
    Uniformly distributed between soma and neurites in hippocampal neurons
  • 参照データベース
  • 別名
    • KIF5A antibody
    • KIF5B antibody
    • KIF5C antibody
    • Kinesin family member 5A antibody
    • Kinesin family member 5B antibody
    • Kinesin family member 5C antibody
    • KINH antibody
    • KINN antibody
    • NKHC antibody
    • NKHC2 antibody
    see all

画像

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Kinesin 5 A + B + C with Anti-Kinesin 5 A + B + C antibody (ab62104) at 5μg/ml.
  • Lane 1 : Anti-Kinesin 5 A + B + C antibody (ab62104) at 0.5 µg/ml
    Lane 2 : Anti-Kinesin 5 A + B + C antibody (ab62104) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate


    Predicted band size : 110 kDa
    Observed band size : 116 kDa (why is the actual band size different from the predicted?)
  • ab62104 at 5 µg/ml staining Kinesin 5 A + B + C in human K562 cells by Immunocytochemistry.

  • Immunofluorescence of KIF5 in K562 cells using ab62104 at 20 ug/ml.

参考文献

This product has been referenced in:
  • Sherman MA  et al. Soluble Conformers of Aß and Tau Alter Selective Proteins Governing Axonal Transport. J Neurosci 36:9647-58 (2016). Read more (PubMed: 27629715) »
  • Su B  et al. Brain-derived neurotrophic factor (BDNF)-induced mitochondrial motility arrest and presynaptic docking contribute to BDNF-enhanced synaptic transmission. J Biol Chem 289:1213-26 (2014). Rat . Read more (PubMed: 24302729) »

See all 2 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Tissue sections (spinal cord)
Specification
spinal cord
Permeabilization
Yes - triton 0.3%
Fixative
Lana's Fix
Username

Abcam user community

Verified customer

投稿 Jul 16 2013

We publish all the data we receive in Abreviews from customers, in same way they submit to us. Customer have used non reducing conditions however we have tested this antibody under reducing/ denaturing conditions so this antibody is fully guaranteed fo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells)
Specification
HeLa Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Oct 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (brain, embryonic E18)
Total protein in input
1000 µg
Specification
brain, embryonic E18
Immuno-precipitation step
Protein G
Username

Lena

Verified customer

投稿 Sep 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain Lysate loaded onto a 5-20% sucrose gradient)
Loading amount
10 µg
Specification
Brain Lysate loaded onto a 5-20% sucrose gradient
Gel Running Conditions
Non-reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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