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Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Kindlin.
(Peptide available as ab69694.)
Our Abpromise guarantee covers the use of ab68041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).|
|IHC-Fr||Use a concentration of 5 µg/ml.|
ab68041 staining Kindlin in adult mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBST (PBS / 0.1% v/v Triton X-100) and blocked with 3% donkey serum / 1% BSA in PBST for 1 hour at 25°C. Samples were incubated with primary antibody (5µg/ml in 1% BSA / 3% donkey serum in PBST) for 48 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Kindlin-positive staining was detected in cells in the epithelial lining of the uterus. Image shows DAPI counterstain, red field (autofluorescence), antibody staining and overlay.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"