• 製品名Anti-Kindlin antibody
    Kindlin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Kindlin
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow, Xenopus laevis, Non Human Primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Kindlin.

    (Peptide available as ab69694.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat - Staurosporine Treated (24hr, 500nM), TE 671, Y79, MEF1, Hela - Staurosporine Treated (24hr, 500nM) It also gave a positive signal in FFPE human liver carcinoma tissue sections


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野



Our Abpromise guarantee covers the use of ab68041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IHC-Fr Use a concentration of 5 µg/ml.


  • 機能Involved in cell adhesion. Contributes to integrin activation. When coexpressed with talin, potentiates activation of ITGA2B. Required for normal keratinocyte proliferation. Required for normal polarization of basal keratinocytes in skin, and for normal cell shape. Required for normal adhesion of keratinocytes to fibronectin and laminin, and for normal keratinocyte migration to wound sites. May mediate TGF-beta 1 signaling in tumor progression.
  • 組織特異性Expressed in brain, skeletal muscle, kidney, colon, adrenal gland, prostate, and placenta. Weakly or not expressed in heart, thymus, spleen, liver, small intestine, bone marrow, lung and peripheral blood leukocytes. Overexpressed in some colon and lung tumors. In skin, it is localized within the epidermis and particularly in basal keratocytes. Not detected in epidermal melanocytes and dermal fibroblasts.
  • 関連疾患Defects in FERMT1 are the cause of Kindler syndrome (KINDS) [MIM:173650]. An autosomal recessive skin disorder characterized by skin blistering, photosensitivity, progressive poikiloderma, and extensive skin atrophy. Additional clinical features include gingival erosions, ocular, esophageal, gastrointestinal and urogenital involvement, and an increased risk of mucocutaneous malignancy. Note=Although most FERMT1 mutations are predicted to lead to premature termination of translation, and to loss of FERMT1 function, significant clinical variability is observed among patients. There is an association of FERMT1 missense and in-frame deletion mutations with milder disease phenotypes, and later onset of complications (PubMed:21936020).
  • 配列類似性Belongs to the kindlin family.
    Contains 1 FERM domain.
    Contains 1 PH domain.
  • ドメインThe FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain. The FERM domain contains the subdomains F1, F2 and F3. It is preceded by a F0 domain with a ubiquitin-like fold. The F0 domain is required for integrin activation and for localization at focal adhesions.
  • 細胞内局在Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > ruffle membrane. Constituent of focal adhesions. Localized at the basal aspect of skin keratinocytes, close to the cell membrane. Colocalizes with filamentous actin. Upon TGFB1 treatment, it localizes to membrane ruffles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C20orf42 antibody
    • Chromosome 20 open reading frame 42 antibody
    • DTGCU 2 antibody
    • DTGCU2 antibody
    • FERM1_HUMAN antibody
    • Fermitin family homolog 1 antibody
    • Fermitin family member 1 antibody
    • Fermt1 antibody
    • FLJ20116 antibody
    • FLJ23423 antibody
    • KIND 1 antibody
    • KIND1 antibody
    • Kinderlin antibody
    • Kindlerin antibody
    • Kindlin 1 antibody
    • Kindlin syndrome protein antibody
    • Kindlin-1 antibody
    • Kindlin1 antibody
    • Unc 112 related protein 1 antibody
    • Unc-112-related protein 1 antibody
    • Unc112 related protein antibody
    • UNC112A antibody
    • URP 1 antibody
    • URP1 antibody
    see all

Anti-Kindlin antibody 画像

  • All lanes : Anti-Kindlin antibody (ab68041) at 1 µg/ml

    Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : Hela Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP)

    Performed under reducing conditions.

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 45 kDa,74 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab68041 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68041, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of Kindlin staining in human liver carcnioma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68041, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab68041 staining Kindlin in adult mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBST (PBS / 0.1% v/v Triton X-100) and blocked with 3% donkey serum / 1% BSA in PBST for 1 hour at 25°C. Samples were incubated with primary antibody (5µg/ml in 1% BSA / 3% donkey serum in PBST) for 48 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    Kindlin-positive staining was detected in cells in the epithelial lining of the uterus. Image shows DAPI counterstain, red field (autofluorescence), antibody staining and overlay.

    See Abreview

Anti-Kindlin antibody (ab68041) 使用論文

This product has been referenced in:
  • Patel H  et al. Kindlin1 regulates microtubule function to ensure normal mitosis. J Mol Cell Biol 8:338-48 (2016). WB ; Human . Read more (PubMed: 26993041) »
  • Natsuga K  et al. Expression of exon-8-skipped kindlin-1 does not compensate for defects of Kindler syndrome. J Dermatol Sci 61:38-44 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21146372) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Uterus)
Permeabilization Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification Uterus
Blocking step 3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde

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投稿 Apr 21 2015