製品の概要

  • 製品名Anti-KIFC1 antibody
    KIFC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KIFC1
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human KIFC1 (NP_002254.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and 293T cells. This antibody gave a positive result when used in the following methanol fixed cell lines: T47D

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.
  • 配列類似性Belongs to the kinesin-like protein family. NCD subfamily.
    Contains 1 kinesin-motor domain.
  • 細胞内局在Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Early endosome. Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HSET antibody
    • HSET KNSL2 antibody
    • KIF C1 antibody
    • KIFC 1 antibody
    • KIFC1 antibody
    • KIFC1_HUMAN antibody
    • Kinesin family member C1 antibody
    • Kinesin like 2 antibody
    • Kinesin like protein 2 antibody
    • Kinesin like protein KIFC1 antibody
    • Kinesin related protein HSET antibody
    • Kinesin-like protein 2 antibody
    • Kinesin-like protein KIFC1 antibody
    • Kinesin-related protein HSET antibody
    • KNSL 2 antibody
    • KNSL2 antibody
    • MGC1202 antibody
    • MGC149736 antibody
    • MGC149737 antibody
    • Mitotic kinesin like protein 2 antibody
    • MKLP 2 antibody
    • MKLP2 antibody
    • RAB6 KIFL antibody
    • Rabkinesin 6 antibody
    see all

Anti-KIFC1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KIFC1 with ab72452 at 1/5000 (0.2µg/ml). Detection: DAB.
  • ICC/IF image of ab72452 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72452 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-KIFC1 antibody (ab72452) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 220 kDa,300 kDa,33 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • HeLa whole cell lysate, (1 mg for IP, 20% of IP loaded) treated with ab72452 at 3µg/mg lysate for IP, and at 1µg/ml for subsequent WB.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-KIFC1 antibody (ab72452) 使用論文

ab72452 has not yet been referenced specifically in any publications.

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