製品の概要

  • 製品名Anti-KIF7 antibody
  • 製品の詳細
    Rabbit polyclonal to KIF7
  • アプリケーション適用あり: WBmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide designed within residues: KRSLCSEGRQ APGNEDELHL APELLWLSPL TEGAPRTREE TRDLVHAPLP L, corresponding to amino acids 1225-1275 of Human KIF7 (NP_940927.2)

  • ポジティブ・コントロール
    • HeLa or 293T cell lysate
  • 特記事項Shelf Life: 1 year from date of receipt

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab95884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 151 kDa.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Acts as both a negative and positive regulator of sonic hedgehog (Shh) pathway, acting downstream of SMO. Negatively regulates the pathway by preventing inappropriate activation of the transcriptional activator GLI2 in the absence of ligand. Positively regulates the pathway by preventing the processing of the transcription factor GLI3 into its repressor form. Required for efficient localization of GLI3 to cilia in response to Shh. May also act as a ciliary motor.
    • 組織特異性Embryonic stem cells, melanotic melanoma and Jurkat T-cells.
    • 配列類似性Belongs to the kinesin-like protein family. KIF27 subfamily.
      Contains 1 kinesin-motor domain.
    • 細胞内局在Cell projection > cilium. SMO is required for its accumulation within cilia. Moves from the cilia base to the cilia tip in response to activation of the Shh pathway.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EQYK340 antibody
      • kif7 antibody
      • KIF7_HUMAN antibody
      • kinesin family member 7 antibody
      • kinesin like protein KIF7 antibody
      • Kinesin-like protein kif7 antibody
      • UNQ340 antibody
      see all

    Anti-KIF7 antibody 画像

    • All lanes : Anti-KIF7 antibody (ab95884) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 151 kDa


      Exposure time : 30 seconds

    Anti-KIF7 antibody (ab95884) 使用論文

    This product has been referenced in:
    • Ho J  et al. Downregulation of the gli transcription factors regulator Kif7 facilitates cell survival and migration of choriocarcinoma cells. PLoS One 9:e108248 (2014). WB ; Human . Read more (PubMed: 25265279) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Mouse Cell lysate - whole cell (MEF)
    Specification MEF
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 03 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"