製品の概要

  • 製品名Anti-KIF5B antibody [KN-03]
    KIF5B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [KN-03] to KIF5B
  • 特異性The antibody reacts with heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. The antibody recognizes kinesin bound to taxol-stabilized microtubules.
  • アプリケーション適用あり: ICC/IF, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
  • 免疫原

    Enriched fraction of porcine brain kinesin.

  • エピトープEpitope mapping has revealed that antibodies ab11882 and ab11883 react with different sets of fragments.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
ICC
  • 追加情報ICC: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes.
    • 配列類似性Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
      Contains 1 kinesin motor domain.
    • ドメインComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
    • 細胞内局在Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Conventional kinesin heavy chain antibody
      • KIF 5B antibody
      • KIF5B antibody
      • Kinesin 1 antibody
      • kinesin 1 (110-120kD) antibody
      • Kinesin 1 heavy chain antibody
      • Kinesin family member 5B antibody
      • Kinesin heavy chain antibody
      • kinesin, heavy chain, ubiquitous antibody
      • Kinesin-1 heavy chain antibody
      • Kinesin1 antibody
      • KINH antibody
      • KINH_HUMAN antibody
      • KNS 1 antibody
      • KNS antibody
      • KNS1 antibody
      • Ubiquitous kinesin heavy chain antibody
      • UKHC antibody
      see all

    Anti-KIF5B antibody [KN-03] 画像

    • ab11883 staining rat neuronal stem cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Saponin, prior to blocking with 10% serum for 1 hour at 21°C.  The primary antibody was diluted 1/500 with PBS + 1% FCS and incubated with the sample for 18 hours at 4°C.  A Cy5® conjugated goat anti-mouse IgG antibody was used as the secondary.

      See Abreview

    Anti-KIF5B antibody [KN-03] (ab11883) 使用論文

    ab11883 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (neuronale stem cells)
    Specification neuronale stem cells
    Fixative Paraformaldehyde
    Permeabilization Yes - 0,5% Saponin
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Dominik Schelshorn

    Verified customer

    投稿 Feb 01 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"