製品の概要

  • 製品名
    Anti-KIF5B antibody [H2]
    KIF5B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [H2] to KIF5B
  • 特異性
    ab23664 recognises the heavy chain of kinesin.
  • アプリケーション
    適用あり: Electron Microscopy, IHC-FoFr, ICC, WB, IP, RIAmore details
  • 種交差性
    交差種: Rat, Chicken, Sea urchin
    交差が予測される動物種: Mammal
  • 免疫原

    Full length protein corresponding to Cow KIF5B.

  • エピトープ
    The epitope recognised by ab23664 is located near the N terminus of Kinesin heavy chain.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 250mM Sodium chloride, 20mM PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    H2
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab23664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Electron Microscopy
IHC-FoFr
ICC
WB
IP
RIA
  • 追加情報
    Electron Microscopy: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    IHC-F: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 109 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes.
    • 配列類似性
      Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
      Contains 1 kinesin motor domain.
    • ドメイン
      Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
    • 細胞内局在
      Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Conventional kinesin heavy chain antibody
      • KIF 5B antibody
      • KIF5B antibody
      • Kinesin 1 antibody
      • kinesin 1 (110-120kD) antibody
      • Kinesin 1 heavy chain antibody
      • Kinesin family member 5B antibody
      • Kinesin heavy chain antibody
      • kinesin, heavy chain, ubiquitous antibody
      • Kinesin-1 heavy chain antibody
      • Kinesin1 antibody
      • KINH antibody
      • KINH_HUMAN antibody
      • KNS 1 antibody
      • KNS antibody
      • KNS1 antibody
      • Ubiquitous kinesin heavy chain antibody
      • UKHC antibody
      see all

    参考文献

    This product has been referenced in:
    • Astanina K & Jacob R KIF5C, a kinesin motor involved in apical trafficking of MDCK cells. Cell Mol Life Sci 67:1331-42 (2010). IP . Read more (PubMed: 20094756) »
    • Zhang L  et al. {gamma}-Diketone Axonopathy: Analyses of Cytoskeletal Motors and Highways in CNS Myelinated Axons. Toxicol Sci 117:180-9 (2010). WB ; Rat . Read more (PubMed: 20554699) »

    See all 3 Publications for this product

    レビューと Q&A

    Thank you for contacting Abcam regarding ab23664.

    I have run the BLAST search using the immunogen sequence for this antibody. I am only pulling up 100% with the heavy chain of kinesin-1. None of the other kinesins come up. I would predict th...

    Read More

    Thank you for your enquiry. The immunogen for this antibody was kinesin isolated from bovine brain. The antibody is described in detail in one of the references listed towards the bottom of the datasheet: Pfister KK, Wagner MC, Stenoien DL, Bra...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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