Anti-KIF5B+KIF5C 抗体 [EPR10276(B)] (ab167429)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10276(B)] to KIF5C + KIF5B
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-KIF5B+KIF5C antibody [EPR10276(B)] -
製品の詳細
Rabbit monoclonal [EPR10276(B)] to KIF5C + KIF5B -
由来種
Rabbit -
特異性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human KIF5B aa 1-100.
Database link: P33176 -
ポジティブ・コントロール
- Human fetal kidney, HeLa, Jurkat and HepG2 lysates; Human brain tissue; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.21% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10276(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167429の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
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ICC/IF |
1/100 - 1/500.
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IP |
1/10 - 1/100.
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特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Predicted molecular weight: 110 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ICC/IF
1/100 - 1/500. |
IP
1/10 - 1/100. |
ターゲット情報
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細胞内局在
KIF5C: Cytoplasm > cytoskeleton. KIF5B: Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons. -
参照データベース
- Entrez Gene: 3799 Human
- Entrez Gene: 3800 Human
- Entrez Gene: 16573 Mouse
- Entrez Gene: 16574 Mouse
- Entrez Gene: 117550 Rat
- Entrez Gene: 311024 Rat
- Omim: 602809 Human
- Omim: 604593 Human
see all
画像
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All lanes : Anti-KIF5B+KIF5C antibody [EPR10276(B)] (ab167429) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) +OE-empty
Lane 2 : 293T (Human embryonic kidney epithelial cell) +OE-mouse KIF5A
Lane 3 : 293T (Human embryonic kidney epithelial cell) +OE-mouse KIF5C
Lane 4 : 293T (Human embryonic kidney epithelial cell) +OE-mouse KIF5B
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 1 secondBlocking and diluting buffer and concentration: 5% NFDM/TBST
ab181602 was used a GAPDH loading control.
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All lanes : Anti-KIF5B+KIF5C antibody [EPR10276(B)] (ab167429) at 1/5000 dilution (purified)
Lane 1 : Jurkat (human acute T cell leukemia) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell ) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 110 kDaBlocking and diluting buffer: 5% NFDM/TBST
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ab167429 (purified) at 1:20 dilution (2μg) immunoprecipitating KIF5B in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab167429 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab167429 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling KIF5B with purified ab167429 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling KIF5B with Purified ab167429 at 1:250 dilution (0.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KIF5B knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab167429 observed at 110 kDa. Red - loading control, ab9484, observed at 37 kDa.
Unpurified ab167429 was shown to specifically react with KIF5B in wild-type cells as signal was lost in KIF5B knockout cells. Wild-type and KIF5B knockout samples were subjected to SDS-PAGE. Ab167429 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/Immunofluorescence analysis HepG2(human hepatocellular carcinoma) labelling KIF5B with purified ab167429 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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All lanes : Anti-KIF5B+KIF5C antibody [EPR10276(B)] (ab167429) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal kidney lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lane 4 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue, labeling KIF5B using unpurified ab167429 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells, labeling KIF5B using unpurified ab167429 at a 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (37)
ab167429 は 37 報の論文で使用されています。
- Xu A et al. Kinesin-1 transports morphologically distinct intracellular virions during vaccinia infection. J Cell Sci 136:N/A (2023). PubMed: 36093836
- Yang X et al. Time-dependent alterations in the rat nigrostriatal system after intrastriatal injection of fibrils formed by α-Syn and tau fragments. Front Aging Neurosci 14:1049418 (2022). PubMed: 36518823
- Charles Jacob HK et al. Identification of novel early pancreatic cancer biomarkers KIF5B and SFRP2 from "first contact" interactions in the tumor microenvironment. J Exp Clin Cancer Res 41:258 (2022). PubMed: 36002889
- Rahmati M & Taherabadi SJ The effects of exercise training on Kinesin and GAP-43 expression in skeletal muscle fibers of STZ-induced diabetic rats. Sci Rep 11:9535 (2021). PubMed: 33953268
- Li R et al. Fibroblast Growth Factor 13 Facilitates Peripheral Nerve Regeneration through Maintaining Microtubule Stability. Oxid Med Cell Longev 2021:5481228 (2021). PubMed: 34457114