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Recombinant fragment corresponding to Human KIF5B. Stalk domain of human KIF5B (amino acids 331-906) expressed in E. coli (FKHC3).
Our Abpromise guarantee covers the use of ab25715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 110 kDa.|
ab25715 staining KIF5B in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.1% Triton and blocked with 0.1% BSA for 16 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS + 0.1% BSA) for 1 hour at 37°C. An Alexa Fluor®546-conjugated donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.