製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to KIF3C
  • アプリケーション
    適用あり: WB, IHC-FoFrmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein corresponding to amino acids 1-793 of Human KIF3C (NP_002245.4).

  • ポジティブ・コントロール
    • HeLa cell lysate and KIF3C transfected 293T cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa.
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Microtubule-based anterograde translocator for membranous organelles.
  • 配列類似性
    Belongs to the kinesin-like protein family. Kinesin II subfamily.
    Contains 1 kinesin-motor domain.
  • 細胞内局在
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA4058 antibody
    • KIF3C antibody
    • KIF3C variant protein antibody
    • KIF3C_HUMAN antibody
    • Kinesin family member 3C antibody
    • Kinesin like protein KIF3C antibody
    • Kinesin-like protein KIF3C antibody
    • mKIAA4058 antibody
    see all

Anti-KIF3C antibody 画像

  • Anti-KIF3C antibody (ab89276) at 1 µg/ml + HeLa cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 89 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-KIF3C antibody (ab89276) at 1 µg/ml

    Lane 1 : KIF3C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 89 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

Anti-KIF3C antibody (ab89276) 使用論文

ab89276 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6.0
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
Yes - 0.3% Triton X-100, 30min, R.T.
Fixative
Paraformaldehyde
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投稿 Aug 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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