製品の概要

  • 製品名
    Anti-KIF3B antibody
  • 製品の詳細
    Mouse polyclonal to KIF3B
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Full length Human KIF3B protein (ENSP00000364864).

  • ポジティブ・コントロール
    • Rat brain tissue lysate. HeLa cell lysate. Cell lysate from transfected 293T cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1 x PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

ターゲット情報

  • 機能
    Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
  • 配列類似性
    Belongs to the kinesin-like protein family. Kinesin II subfamily.
    Contains 1 kinesin-motor domain.
  • 細胞内局在
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HH0048 antibody
    • KIAA0359 antibody
    • KIF3B antibody
    • KIF3B_HUMAN antibody
    • Kinesin family member 3B antibody
    • Kinesin like protein KIF3B antibody
    • Kinesin-like protein KIF3B antibody
    • Microtubule plus end directed kinesin motor 3B antibody
    • Microtubule plus end-directed kinesin motor 3B antibody
    • Similar to Mouse kinesin KIF3B antibody
    see all

画像

  • Anti-KIF3B antibody (ab89278) at 1 µg/ml + Rat brain tissue lysate at 50 µg

    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • Anti-KIF3B antibody (ab89278) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • All lanes : Anti-KIF3B antibody (ab89278) at 1 µg/ml

    Lane 1 : cell lysate from KIF3B transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 85 kDa
    Observed band size : 85 kDa

プロトコール

参考文献

ab89278 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-20% TG)
Sample
Mouse Tissue lysate - whole (retina and brain)
Specification
retina and brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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投稿 Mar 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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