Anti-KIF22 抗体 [EP2748] (ab108535)
Key features and details
- Rabbit monoclonal [EP2748] to KIF22
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
-
製品名
Anti-KIF22 antibody [EP2748]
KIF22 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2748] to KIF22 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- HEK-293T, HeLa, MCF7, and SH-SY5Y cell lysates
-
特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP2748 -
アイソタイプ
IgG -
研究分野
関連製品
-
Isotype control
-
KO cell lines
-
KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108535の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 73 kDa. |
ターゲット情報
-
機能
Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA. -
配列類似性
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
翻訳後修飾
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton. - Information by UniProt
-
参照データベース
- Entrez Gene: 3835 Human
- Omim: 603213 Human
- SwissProt: Q14807 Human
- Unigene: 612151 Human
-
別名
- A 328A3.2 antibody
- KID antibody
- KIF 22 antibody
see all
画像
-
All lanes : Anti-KIF22 antibody [EP2748] (ab108535) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : KIF22 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab108535 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108535 was shown to react with KIF22 in HEK-293T wild-type cells in western blot with loss of signal observed in KIF22 knockout cell line ab266817 (KIF22 knockout cell lysate ab258018). Wild-type and KIF22 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-KIF22 antibody [EP2748] (ab108535) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 73 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab108535 は論文での使用が確認できていません。