製品の概要

  • 製品名
    Anti-KIF22 antibody [EP2747Y]
    KIF22 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2747Y] to KIF22
  • アプリケーション
    適用あり: WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues on the N-terminus of KIF22

  • ポジティブ・コントロール
    • HeLa cell lysate
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP2747Y
  • アイソタイプ
    IgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab75783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
IP 1/10.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
  • 配列類似性
    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • 翻訳後修飾
    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A 328A3.2 antibody
    • KID antibody
    • KIF 22 antibody
    • kif22 antibody
    • KIF22_HUMAN antibody
    • Kinesin family member 22 antibody
    • Kinesin like 4 antibody
    • Kinesin like DNA binding protein antibody
    • Kinesin like DNA binding protein pseudogene antibody
    • Kinesin like protein 4 antibody
    • Kinesin like protein KIF22 antibody
    • Kinesin-like DNA-binding protein antibody
    • Kinesin-like protein 4 antibody
    • Kinesin-like protein KIF22 antibody
    • KNSL 4 antibody
    • KNSL4 antibody
    • OBP 1 antibody
    • OBP 2 antibody
    • OBP antibody
    • OBP1 antibody
    • OBP2 antibody
    • Origin of plasmid DNA replication binding protein antibody
    • OriP binding protein antibody
    • OTTHUMP00000123406 antibody
    • SEMDJL2 antibody
    see all

Anti-KIF22 antibody [EP2747Y] 画像

  • Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labelled oat anti rabbit at 1/2000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa

Anti-KIF22 antibody [EP2747Y] (ab75783) 使用論文

This product has been referenced in:
  • Li C  et al. NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force. Nat Commun 7:10597 (2016). IF . Read more (PubMed: 26839278) »

See 1 Publication for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - nuclear (cartilage)
Loading amount
10 µg
Specification
cartilage
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

投稿 Oct 26 2012

Thank you for contacting us and for your enquiry. As far as we are aware, non of our KIF22 antibodies has been tested with samples from zebrafish. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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