Anti-KIF22 抗体 (ab56235)

製品の概要

  • 製品名Anti-KIF22 antibody
    KIF22 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KIF22
  • アプリケーション適用あり: WB, ELISAmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: MVLMKTVEEK DLEIERLKTK QKELEAKMLA QKAEEKENHC PTMLRPLSHR, corresponding to C terminal amino acids 469-518 of Human KIF22

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab56235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 73 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

  • 追加情報Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
    • 配列類似性Belongs to the kinesin-like protein family.
      Contains 1 kinesin-motor domain.
    • 翻訳後修飾Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A 328A3.2 antibody
      • KID antibody
      • KIF 22 antibody
      • kif22 antibody
      • KIF22_HUMAN antibody
      • Kinesin family member 22 antibody
      • Kinesin like 4 antibody
      • Kinesin like DNA binding protein antibody
      • Kinesin like DNA binding protein pseudogene antibody
      • Kinesin like protein 4 antibody
      • Kinesin like protein KIF22 antibody
      • Kinesin-like DNA-binding protein antibody
      • Kinesin-like protein 4 antibody
      • Kinesin-like protein KIF22 antibody
      • KNSL 4 antibody
      • KNSL4 antibody
      • OBP 1 antibody
      • OBP 2 antibody
      • OBP antibody
      • OBP1 antibody
      • OBP2 antibody
      • Origin of plasmid DNA replication binding protein antibody
      • OriP binding protein antibody
      • OTTHUMP00000123406 antibody
      see all

    Anti-KIF22 antibody 画像

    • Anti-KIF22 antibody (ab56235) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 73 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.

    Anti-KIF22 antibody (ab56235) 使用論文

    ab56235 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us and for your enquiry. As far as we are aware, non of our KIF22 antibodies has been tested with samples from zebrafish. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
    Username

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    Verified customer

    投稿 Dec 16 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"