製品の概要

  • 製品名Anti-KIF20A antibody
    KIF20A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KIF20A
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 840 and the C-terminus (residue 890) of human KIF20A. (NP_005724.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
IP
  • 追加情報IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.
    • 配列類似性Belongs to the kinesin-like protein family.
      Contains 1 kinesin-motor domain.
    • 翻訳後修飾Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.
    • 細胞内局在Golgi apparatus. Cytoplasm > cytoskeleton > spindle.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AA415432 antibody
      • FLJ21151 antibody
      • GG10 2 antibody
      • GG10_2 antibody
      • KI20A_HUMAN antibody
      • KIF20A antibody
      • kinesin family member 20A antibody
      • Kinesin like protein KIF20A antibody
      • Kinesin-like protein 174 antibody
      • Kinesin-like protein KIF20A antibody
      • mitotic kinesin like protein 2 antibody
      • Mitotic kinesin-like protein 2 antibody
      • MKlp2 antibody
      • OTTHUMP00000159481 antibody
      • RAB6 interacting, kinesin like (rabkinesin6) antibody
      • Rab6-interacting kinesin-like protein antibody
      • RAB6-INTERACTING PROTEIN, KINESIN-LIKE antibody
      • RAB6KIFL antibody
      • Rabkinesin 6 antibody
      • Rabkinesin-6 antibody
      see all

    Anti-KIF20A antibody 画像

    • IHC image of KIF20A staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70791, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-KIF20A antibody (ab70791) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 100 kDa
      Observed band size : 100 kDa
      Additional bands at : 120 kDa,31 kDa,60 kDa,68 kDa,73 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab70791 at 1 µg/ml for WB, subsequent to using for IP at 3 µg/mg lysate.
      Lane 2: IgG control.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-KIF20A antibody (ab70791) 使用論文

    ab70791 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab70791.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"