The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa).
Use at 2-5 µg/mg of lysate.
関連性KIF1C belongs to the kinesin-like protein family, Unc-104 subfamily. It contains one FHA domain and one kinesin-motor domain. It is the motor required for the retrograde transport of Golgi vesicles to the endoplasmic reticulum. KIF1C has a microtubule plus end-directed motility. It is expressed in all tissues examined, with most abundant expression in heart and skeletal muscle.
Immunoprecipitation/ Western Blot of KIF1C.
Lane 1: ab72238 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72238 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
IHC image of ab72238 staining in human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72238, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-KIF1C antibody (ab72238) 使用論文
This product has been referenced in:
Jones LA et al. Dynein light intermediate chains maintain spindle bipolarity by functioning in centriole cohesion. J Cell Biol207:499-516 (2014).
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