Rabbit polyclonal to KIF1C
Recombinant fragment, corresponding to a region within amino acids 452-718 of Human KIF1C (O43896).
A549, HepG2 and HCT116 whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
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Immunogen affinity purified
is purified by antigen-affinity chromatography.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/3000. Predicted molecular weight: 123 kDa.
Motor required for the retrograde transport of Golgi vesicles to the endoplasmic reticulum. Has a microtubule plus end-directed motility.
Expressed in all tissues examined, with most abundant expression in heart and skeletal muscle.
Spastic ataxia 2, autosomal recessive
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Unc-104 subfamily.
Contains 1 FHA domain. Contains 1 kinesin motor domain.
Phosphorylated on tyrosine residues.
Information by UniProt
Kinesin family member 1C antibody
Immunocytochemistry/ Immunofluorescence - Anti-KIF1C antibody (ab125903) This image is courtesy of an anonymous Abreview
ab125903 staining KIF1C in Human skin fibroblast cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 5% BSA and 0.1% Triton) for 18 hours at 4°C. An Cy2®-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Western blot - Anti-KIF1C antibody (ab125903)
Anti-KIF1C antibody (ab125903) at 1/500 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size :
5% SDS PAGE
Secondary antibody -
anti-rabbit HRP ( ab6721)
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