製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KIF1B
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 of Human KIF1B.

    (Peptide available as ab74827.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysate: Mouse Brain, 0 days old.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 204 kDa).

ターゲット情報

  • 関連性
    KIF1B, or kinesin-like protein (Klp) functions as a motor for mitochondrial transport, and has a microtubule plus end-directed motility. The KIF1B beta isoform is abundant in brain, while the alpha isoform is abundant in skeletal muscle. Mutations in the KIF1B gene are the cause of Charcot-Marie-Tooth disease type 2A1, which is a primary peripheral axon neuropathy. The KIF1B beta isoform is down-regulated in sporadic amyotrophic lateral sclerosis (ALS).
  • 細胞内局在
    Cytoplasmic and Mitochondrial
  • 参照データベース
  • 別名
    • Charcot Marie Tooth neuropathy 2A antibody
    • CMT 2 antibody
    • CMT 2A antibody
    • CMT 2A1 antibody
    • CMT2 A antibody
    • CMT2 A1 antibody
    • CMT2 antibody
    • CMT2A 1 antibody
    • CMT2A antibody
    • CMT2A1 antibody
    • D4Mil1e antibody
    • HMSN II antibody
    • HMSNII antibody
    • HMSNII, hereditary motor sensory neuropathy II antibody
    • KIF 1B antibody
    • KIF1 B antibody
    • KIF1B p130 antibody
    • KIF1B p204 antibody
    • KIF1Bp130 antibody
    • KIF1Bp204 antibody
    • kinesin family member 1B antibody
    • Kinesin like protein KIF1B antibody
    • Klp antibody
    see all

画像

  • Anti-KIF1B antibody (ab69614) at 2 µg/ml + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 204 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 230 kDa,45 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab69614 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69614, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab69614 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (DRG)
Specification
DRG
Fixative
Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

投稿 Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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