Anti-KIF18A 抗体 (ab72417)
Key features and details
- Rabbit polyclonal to KIF18A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIF18A antibody
KIF18A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF18A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residue 848 and the C-terminus (residue 898) of human KIF18A (NP_112494.2)
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ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab72417 was affinity purified using an epitope specific to KIF18A immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72417の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Microtubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression. -
配列類似性
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
翻訳後修飾
Glycosylated.
Ubiquitinated. -
細胞内局在
Cell projection > ruffle. Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 81930 Human
- Omim: 611271 Human
- SwissProt: Q8NI77 Human
- Unigene: 301052 Human
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別名
- AU024633 antibody
- B130001M12Rik antibody
- DKFZp434G2226 antibody
see all
画像
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All lanes : Anti-KIF18A antibody (ab72417) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of KIF18A by Western Blot of Immunprecipitate.
ab72417 at 1µg/ml staining KIF18A in HeLa whole cell lysate immunoprecipitated using ab72417 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab72417 は 1 報の論文で使用されています。
- Liu G et al. KIF18A promotes head and neck squamous cell carcinoma invasion and migration via activation of Akt signaling pathway. Transl Cancer Res 8:2252-2263 (2019). PubMed: 35116978