製品の概要

  • 製品名Anti-KIF14 antibody
    KIF14 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KIF14
  • アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14) encoded within exon 16.

  • ポジティブ・コントロール
    • HeLa whole cell extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 190 kDa.
IP Use at 1-4 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC 1/250 - 1/1000.

ターゲット情報

  • 機能Plays an essential role in cytokinesis.
  • 配列類似性Belongs to the kinesin-like protein family.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • 発生段階Up-regulated in cells progressing through G2/M phase.
  • 細胞内局在Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0042 antibody
    • KIF 14 antibody
    • KIF14 antibody
    • KIF14_HUMAN antibody
    • Kinesin Family Member 14 antibody
    • Kinesin like protein KIF14 antibody
    • Kinesin-like protein KIF14 antibody
    • MGC142302 antibody
    see all

Anti-KIF14 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling KIF14 with ab3747 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 190 kDa

    Sample: HeLa whole cell lysate

    1) WB - 50µg

    2) IP - 1mg

    Antibody: Affinity purified Rabbit anti-KIF (ab3747)

    1) & 2) WB - 1 / 4000 dilution.

    2) IP - concentration of 3µg / mg of lysate.

    Detection: ECL.

    Sample: HeLa whole cell lysate

    1) WB - 50µg

    2) IP - 1mg

    Antibody: Affinity purified Rabbit anti-KIF (ab3747)

    1) & 2) WB - 1 / 4000 dilution.

    2) IP - concentration of 3µg / mg of lysate.

    Detection: ECL.

  • Whole cell lysate (50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. ab3747 used for western blot at 1 µg/ml and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.

    Lane 1 - Input.

    Lane 2 - IP.

    Lane 3 - Control IgG IP.

Anti-KIF14 antibody (ab3747) 使用論文

ab3747 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab3747.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"