製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KIF13A
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1725 and 1775 of human KIF13A (NP_071396.3)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab72415 was affinity purified using an epitope specific to KIF13A immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 238 kDa (predicted molecular weight: 203 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Plus end-directed microtubule-dependent motor protein involved in intracellular transport and regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis and cytokinesis. Mediates the transport of M6PR-containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. During melanosome maturation, required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. Also required for the abcission step in cytokinesis: mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis.
  • 組織特異性
    Widely expressed, with highest levels in heart, brain and skeletal muscle.
  • 配列類似性
    Belongs to the kinesin-like protein family.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • 発生段階
    Expressed at very low level in most fetal tissues, but at higher levels in skeletal muscle and lung.
  • 細胞内局在
    Cytoplasm > cytoskeleton > centrosome. Midbody. Endosome membrane. Golgi apparatus membrane. Recruited to the midbody during cytokinesis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA500C11.2 antibody
    • FLJ27232 antibody
    • Homolog of mouse KIF13A mannose 6 phosphate receptor transporter antibody
    • KI13A_HUMAN antibody
    • KIF 13A antibody
    • Kif13a antibody
    • Kinesin family member 13A antibody
    • Kinesin like protein KIF13A antibody
    • Kinesin like protein RBKIN antibody
    • Kinesin-like protein KIF13A antibody
    • Kinesin-like protein RBKIN antibody
    • RBKIN antibody
    see all

画像

  • All lanes : Anti-KIF13A antibody (ab72415) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 203 kDa
    Observed band size : 238 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of KIF13A by Western Blot of Immunprecipitate.
    ab72415 at 1µg/ml staining KIF13A in HeLa whole cell lysate immunoprecipitated using ab72415 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

参考文献

This product has been referenced in:
  • Yamada KH  et al. KIF13B regulates angiogenesis through Golgi to plasma membrane trafficking of VEGFR2. J Cell Sci 127:4518-30 (2014). Read more (PubMed: 25128562) »

See 1 Publication for this product

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