The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 238 kDa (predicted molecular weight: 203 kDa).
Use at 2-5 µg/mg of lysate.
Plus end-directed microtubule-dependent motor protein involved in intracellular transport and regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis and cytokinesis. Mediates the transport of M6PR-containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. During melanosome maturation, required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. Also required for the abcission step in cytokinesis: mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis.
Widely expressed, with highest levels in heart, brain and skeletal muscle.
Belongs to the kinesin-like protein family. Contains 1 FHA domain. Contains 1 kinesin-motor domain.
Expressed at very low level in most fetal tissues, but at higher levels in skeletal muscle and lung.
Cytoplasm > cytoskeleton > centrosome. Midbody. Endosome membrane. Golgi apparatus membrane. Recruited to the midbody during cytokinesis.
Detection of KIF13A by Western Blot of Immunprecipitate.
ab72414, at 1µg/ml, staining KIF13A in HeLa whole cell lysate immunoprecipitated using ab72414 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.