Anti-KIF13A 抗体 (ab72414)
Key features and details
- Rabbit polyclonal to KIF13A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIF13A antibody -
製品の詳細
Rabbit polyclonal to KIF13A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat -
免疫原
Synthetic peptide corresponding to a region between residues 1675 and 1725 of human KIF13A (NP_071396.3)
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ポジティブ・コントロール
- HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab72414 was affinity purified using an epitope specific to KIF13A immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72414の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 238 kDa (predicted molecular weight: 203 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 238 kDa (predicted molecular weight: 203 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Plus end-directed microtubule-dependent motor protein involved in intracellular transport and regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis and cytokinesis. Mediates the transport of M6PR-containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. During melanosome maturation, required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. Also required for the abcission step in cytokinesis: mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis. -
組織特異性
Widely expressed, with highest levels in heart, brain and skeletal muscle. -
配列類似性
Belongs to the kinesin-like protein family.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain. -
発生段階
Expressed at very low level in most fetal tissues, but at higher levels in skeletal muscle and lung. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. Midbody. Endosome membrane. Golgi apparatus membrane. Recruited to the midbody during cytokinesis. - Information by UniProt
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参照データベース
- Entrez Gene: 63971 Human
- Omim: 605433 Human
- SwissProt: Q9H1H9 Human
- Unigene: 94499 Human
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別名
- bA500C11.2 antibody
- FLJ27232 antibody
- Homolog of mouse KIF13A mannose 6 phosphate receptor transporter antibody
see all
画像
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All lanes : Anti-KIF13A antibody (ab72414) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 203 kDa
Observed band size: 238 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of KIF13A by Western Blot of Immunprecipitate.
ab72414, at 1µg/ml, staining KIF13A in HeLa whole cell lysate immunoprecipitated using ab72414 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab72414 は論文での使用が確認できていません。