製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 220 kDa.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. May play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration (By similarity). Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway.
  • 組織特異性Abundant in developing and adult neural tissues as well as neuroendocrine cells and dendritic cells. Overexpressed in melanoma and melanoma cell lines.
  • 配列類似性Contains 12 ANK repeats.
    Contains 1 KAP NTPase domain.
  • ドメインThe transmembrane domain mediates interaction with NTRK1.
  • 翻訳後修飾Tyrosine phosphorylated by NTRK1, NTRK2, EPHB2 and EPHA4. Phosphorylation at Ser-918 is induced by phorbol ester treatment. Phosphorylation by NTRK2 is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. Phosphorylation by NTRK1 is induced by nerve growth factor (NGF).
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ankyrin repeat-rich membrane-spanning protein antibody
    • arms antibody
    • KDIS_HUMAN antibody
    • kidins220 antibody
    • Kinase D interacting substance of 220 kDa antibody
    • Kinase D-interacting substrate of 220 kDa antibody
    • rgd 619949 antibody
    see all

Anti-Kidins220 antibody 画像

  • ICC/IF image of ab34790 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34790, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Kidins220 antibody (ab34790) 使用論文

This product has been referenced in:
  • Cesca F  et al. Functional Interaction between the Scaffold Protein Kidins220/ARMS and Neuronal Voltage-Gated Na+ Channels. J Biol Chem 290:18045-55 (2015). Read more (PubMed: 26037926) »
  • Li J  et al. PI3K p110a/Akt signaling negatively regulates secretion of the intestinal peptide neurotensin through interference of granule transport. Mol Endocrinol 26:1380-93 (2012). Human . Read more (PubMed: 22700584) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us. I am sorry we are unable to provide western blot images for anti Borrelia antibodies. I would suggest checking the Swissprot website for checking the molecular weight of unprocessed protein. http://www.uniprot.org/unipr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"