製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KIAA1967
  • アプリケーション
    適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide from a region between residue 875 and the C-terminus (residue 923) of Human KIAA1967 (UniProt: Q8N163).

  • ポジティブ・コントロール
    • Nuclear extract from HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
IP Use at 1-10 µg/mg of lysate.

ターゲット情報

  • 機能
    Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity.
  • 組織特異性
    Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCAR2 antibody
    • Cell cycle and apoptosis regulator 2 antibody
    • Cell division cycle and apoptosis regulator protein 2 antibody
    • DBC-1 antibody
    • DBC.1 antibody
    • DBC1 antibody
    • DBIRD complex subunit KIAA1967 antibody
    • Deleted in breast cancer 1 antibody
    • Deleted in breast cancer gene 1 protein antibody
    • K1967_HUMAN antibody
    • KIAA1967 antibody
    • NET35 antibody
    • p30 DBC antibody
    • p30 DBC protein antibody
    • p30DBC antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling KIAA1967 with ab70242 at 1/1000 (1µg/ml). Detection: DAB.
  • Lanes 1 - 2 : Anti-KIAA1967 antibody (ab70242) at 1/1000 dilution
    Lanes 3 - 4 : Anti-KIAA1967 antibody (ab70242) at 1/10000 dilution

    Lane 1 : Nuclear extract from HeLa cells at 15 µg
    Lane 2 : Nuclear extract from HeLa cells at 45 µg
    Lane 3 : Nuclear extract from HeLa cells at 15 µg
    Lane 4 : Nuclear extract from HeLa cells at 45 µg

    Developed using the ECL technique

    Predicted band size : 103 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds
  • Immunoprecipitation: ab70242 used at 1 µg/100 µg of nuclear extract from HeLa cells. Western blot: 1/1000 ab70242. Detection: Chemiluminescence with exposure times of 5 mins

参考文献

This product has been referenced in:
  • Raynes R  et al. The SIRT1 modulators AROS and DBC1 regulate HSF1 activity and the heat shock response. PLoS One 8:e54364 (2013). WB ; Human . Read more (PubMed: 23349863) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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