The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/20000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
Use at 1-10 µg/mg of lysate.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity.
Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling KIAA1967 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-KIAA1967 antibody (ab70239)
All lanes : Anti-KIAA1967 antibody (ab70239) at 1/10000 dilution
Lane 1 : HeLa cell nuclear extract at 15 µg Lane 2 : HeLa cell nuclear extract at 45 µg