製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to KIAA1199
  • アプリケーション
    適用あり: ICC/IF, ELISA, WBmore details
  • 種交差性
    交差種: Human, Recombinant fragment
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant tagged fragment: LYDGPINIQN CTFRKFVALE GRHTSALAFR LNNAWQSCPH NNVTGIAFED VPITSRVFFG EPGPWFNQLD MDGDKTSVFH DVDGSVSEYP GSYLTKNDNW , corresponding to amino acids 880-980 of Human KIAA1199

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
ELISA
WB
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 153 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      KIAA1199 belongs to the TMEM2 family and contains 4 PbH1 repeats. It is expressed in the inner ear. It is expressed in cochlea and vestibule tissues and it may be involved in hearing. Defects in KIAA1199 may be a cause of non-syndromic hearing loss. It is also thought that KIAA1199 is a tumor suppressor that may be involved in establishing cellular mortality in normal human cells.
    • 参照データベース
    • 別名
      • IR2155535 antibody
      • KIAA1199 protein antibody
      • OTTHUMP00000185569 antibody
      • OTTHUMP00000195036 antibody
      • TMEM2L antibody
      • Transmembrane protein 2 like antibody
      see all

    Anti-KIAA1199 antibody 画像

    • Anti-KIAA1199 antibody (ab76849) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 153 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    Anti-KIAA1199 antibody (ab76849) 使用論文

    This product has been referenced in:
    • Jia S  et al. KIAA1199 promotes migration and invasion by Wnt/ß-catenin pathway and MMPs mediated EMT progression and serves as a poor prognosis marker in gastric cancer. PLoS One 12:e0175058 (2017). IHC-P ; Human . Read more (PubMed: 28422983) »
    • Soroosh A  et al. Crohn's Disease Fibroblasts Overproduce the Novel Protein KIAA1199 to Create Proinflammatory Hyaluronan Fragments. Cell Mol Gastroenterol Hepatol 2:358-368.e4 (2016). Read more (PubMed: 27981209) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human recombinant protein of KIAA1199 in COS-1 cel)
    Specification
    human recombinant protein of KIAA1199 in COS-1 cel
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.25% Triton X-100 in PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
    Username

    Mr. Cem Kuscu

    Verified customer

    投稿 Dec 29 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (COS-1 with recombinant KIAA1199,and cell lysates f)
    Loading amount
    30 µg
    Specification
    COS-1 with recombinant KIAA1199,and cell lysates f
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Mr. Cem Kuscu

    Verified customer

    投稿 Dec 29 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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