製品の概要

  • 製品名Anti-KIAA0528 antibody
    KIAA0528 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KIAA0528
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Sea Urchin, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residue 375 and 425 of human KIAA0528 ( NP_055617.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab80470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 110 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性KIAA0528 is a protein that was first identified in the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. The function of KIAA0528 has not been characterized. By similarity KO528 contains a C2 domain, a domain that is found in proteins that bind phospholipids. The C2 domain is also present in a family of proteins involved in synaptic vesicle trafficking; thus, the function of KIAA0528 is predicted to be involved in intracellular transport.
  • 参照データベース
  • 別名
    • DKFZp779N2044 antibody
    • Hypothetical protein LOC9847 antibody
    • Uncharacterized protein KIAA0528 antibody

Anti-KIAA0528 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labelling KIAA0528 with ab80470 at 1/400 (2.5µg/ml). Detection: DAB.
  • All lanes : Anti-KIAA0528 antibody (ab80470) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 110 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
  • Lane 1: ab80470, at 1 µg/mg, staining KIAA0528 in HeLa whole cell lysate by Western blot of immunoprecipitate (1 mg of lysate for IP, 20% of IP loaded). Immunoprecipitation was performed by using ab80470 at 3 µg/mg of lysate.
    Lane 2: Control IgG.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-KIAA0528 antibody (ab80470) 使用論文

ab80470 has not yet been referenced specifically in any publications.

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