製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KIAA0513
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen, corresponding to amino acids 134-271 of Human KIAA0513.

  • ポジティブ・コントロール
    • KIAA0513-transfected HEK293T lysate; Human cerebellum tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

  • 組織特異性
    Widely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • K0513_HUMAN antibody
    • KIAA0513 antibody
    • Uncharacterized protein KIAA0513 antibody

画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121382 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-KIAA0513 antibody (ab121382) at 1/250 dilution

    Lane 1 : vector only transfected HEK293T lysate
    Lane 2 : KIAA0513-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique10-20% SDS PAGE
  • ab121382 at 1/500 dilution staining KIAA0513 in Paraffin-embedded Human cerebellum tissue by Immunohistochemistry. Note: strong cytoplasmic and nuclear positivity in Purkinje cells and strong cytoplasmic positivity in cells in granular layer and molecular layer.

参考文献

ab121382 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (cerebellum, cortical, hippocampal)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
cerebellum, cortical, hippocampal
Blocking step
1x Odyssey blocking buffer(OBB) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
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投稿 Nov 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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