製品の概要

  • 製品名Anti-KIAA0427 antibody
    KIAA0427 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KIAA0427
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (QVQGLLADKT EGDGESERTQ SHISQWTADC SEPLDSSCSF SRGRAPPQQN) of Human KIAA0427 (NP_055587).

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

ターゲット情報

  • 機能Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD).
  • 組織特異性Widely expressed.
  • 配列類似性Belongs to the CTIF family.
    Contains 1 MIF4G domain.
  • 細胞内局在Cytoplasm > perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CBP80/20-dependent translation initiation factor antibody
    • ctif antibody
    • CTIF_HUMAN antibody
    • Gm672 antibody
    • Hypothetical protein LOC9811 antibody
    • Uncharacterized protein KIAA0427 antibody
    see all

Anti-KIAA0427 antibody 画像

  • Anti-KIAA0427 antibody (ab82891) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

Anti-KIAA0427 antibody (ab82891) 使用論文

ab82891 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"