製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KIAA0319
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 151-200 (EEMSEYSDDY RELEKDLLQP SGKQEPRGSA EYTDWGLLPG SEGAFNSSVG) of Human KIAA0319 (EAW55455).

  • ポジティブ・コントロール
    • HepG2 cell lysate; Human kidney tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Constituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    Involved in neuronal migration during development of the cerebral neocortex. May function in a cell autonomous and a non-cell autonomous manner and play a role in appropriate adhesion between migrating neurons and radial glial fibers. May also regulate growth and differentiation of dendrites.
  • 組織特異性
    Detected in adult brain cortex and fetal frontal lobe (at protein level). Highly expressed in brain cortex, putamen, amygdala, hippocampus and cerebellum.
  • 関連疾患
    Defects in KIAA0319 may be a cause of susceptibility to dyslexia type 2 (DYX2) [MIM:600202]; also known as specific reading disability type 2. Dyslexia is a relatively common, complex cognitive disorder that affects 5% to 10% of school-aged children. The disorder is characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities and in the absence of sensory or neurological disability. Note=A lower expression is associated with the risk haplotype.
  • 配列類似性
    Contains 1 MANSC domain.
    Contains 5 PKD domains.
  • 発生段階
    Expressed in the developing cerebral neocortex and glanglionic eminence in 57 days post-fertilization fetal brain.
  • 翻訳後修飾
    N-glycosylated.
    O-glycosylated.
    Shedding of the extracellular domain and intramembrane cleavage produce several proteolytic products. The intramembrane cleavage releases a soluble cytoplasmic polypeptide that translocates to the nucleolus.
  • 細胞内局在
    Cell membrane. Early endosome membrane. Low-abundance isoforms lacking the transmembrane domain have been described; these are secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DLX 2 antibody
    • DLX2 antibody
    • DYLX 2 antibody
    • DYLX2 antibody
    • Dyslexia susceptibility 2 antibody
    • Dyslexia-associated protein KIAA0319 antibody
    • DYX 2 antibody
    • DYX2 antibody
    • K0319_HUMAN antibody
    • Kiaa0319 antibody
    • MGC176717 antibody
    see all

画像

  • Anti-KIAA0319 antibody (ab118923) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 56 kDa
  • ab118923, at 8 µg/ml, staining KIAA0319 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    Cells with positive label are epithelial cells of renal tubules (indicated by arrows).

参考文献

ab118923 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-10 % gradient gel)
Loading amount
100 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jul 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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