製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KHSRP
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human KHSRP.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human whole cell lysates: HeLa; HEK293; MCF7; HepG2.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 mg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa).

ターゲット情報

  • 機能
    Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
  • 組織特異性
    Detected in neural and non-neural cell lines.
  • 配列類似性
    Belongs to the KHSRP family.
    Contains 4 KH domains.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Far upstream element-binding protein 2 antibody
    • FBP2 antibody
    • FUBP2 antibody
    • FUBP2_HUMAN antibody
    • FUSE binding protein 2 antibody
    • FUSE-binding protein 2 antibody
    • KH type splicing regulatory protein (FUSE binding protein 2) antibody
    • KH type splicing regulatory protein antibody
    • KH type-splicing regulatory protein antibody
    • KHSRP antibody
    • KSRP antibody
    • p75 antibody
    see all

画像

  • All lanes : Anti-KHSRP antibody (ab95229) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 88 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

プロトコール

参考文献

ab95229 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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