製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KHSRP
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human, Zebrafish
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide within residues: WEEYYKKQAQ VATGGGPGAP PGSQPDYSAA WAEYYRQQAA YYGQTPGPGG , corresponding to internal sequence amino acids 648-697 of Human KHSRP (NP_003676)

  • ポジティブ・コントロール
    • 293T cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).Can be blocked with Human KHSRP peptide (ab111652). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
  • 組織特異性
    Detected in neural and non-neural cell lines.
  • 配列類似性
    Belongs to the KHSRP family.
    Contains 4 KH domains.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Far upstream element-binding protein 2 antibody
    • FBP2 antibody
    • FUBP2 antibody
    • FUBP2_HUMAN antibody
    • FUSE binding protein 2 antibody
    • FUSE-binding protein 2 antibody
    • KH type splicing regulatory protein (FUSE binding protein 2) antibody
    • KH type splicing regulatory protein antibody
    • KH type-splicing regulatory protein antibody
    • KHSRP antibody
    • KSRP antibody
    • p75 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling KHSRP with ab83291 at 4.0-8.0µg/ml. Positive staining shown in cardiac cells. Magnification: 400X.
  • Anti-KHSRP antibody (ab83291) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • ICC/IF image of ab83291 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83291, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-KHSRP antibody (ab83291) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 10 µg
    Lane 4 : Zebrafish liver homogenate at 10 µg
    Lane 5 : Zebrafish skeletal muscle homogenate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 73 kDa


    Exposure time : 5 minutes

参考文献

This product has been referenced in:
  • Adachi S  et al. ZFP36L1 and ZFP36L2 control LDLR mRNA stability via the ERK-RSK pathway. Nucleic Acids Res 42:10037-49 (2014). Read more (PubMed: 25106868) »
  • Hudy MH & Proud D Cigarette smoke enhances human rhinovirus-induced CXCL8 production via HuR-mediated mRNA stabilization in human airway epithelial cells. Respir Res 14:88 (2013). Read more (PubMed: 23988199) »

See all 3 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab83291.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録