製品の概要

  • 製品名Anti-KHSRP antibody
    KHSRP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to KHSRP
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: VPDGMVGLII GRGGEQINKI QQDSGCKVQI SPDSGGLPER SVSLTGAPES VQKAKMMLDD IVSRGRGGPP GQFHDNANGG QNGTVQEIM, corresponding to amino acids 151-240 of Human KHSRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab56438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. However this antibody detects endogenous protein in IHC-P.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
  • 組織特異性Detected in neural and non-neural cell lines.
  • 配列類似性Belongs to the KHSRP family.
    Contains 4 KH domains.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Far upstream element-binding protein 2 antibody
    • FBP2 antibody
    • FUBP2 antibody
    • FUBP2_HUMAN antibody
    • FUSE binding protein 2 antibody
    • FUSE-binding protein 2 antibody
    • KH type splicing regulatory protein (FUSE binding protein 2) antibody
    • KH type splicing regulatory protein antibody
    • KH type-splicing regulatory protein antibody
    • KHSRP antibody
    • KSRP antibody
    • p75 antibody
    see all

Anti-KHSRP antibody 画像

  • KHSRP antibody (ab56438) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human liver.
  • Western blot against tagged recombinant protein immunogen using ab56438 KHSRP antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
  • ICC/IF image of ab56438 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56438, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-KHSRP antibody (ab56438) 使用論文

This product has been referenced in:
  • Xie LQ  et al. Novel proteomic strategy reveal combined alpha1 antitrypsin and cathepsin D as biomarkers for colorectal cancer early screening. J Proteome Res 9:4701-9 (2010). WB ; Human . Read more (PubMed: 20666480) »
  • Antrobus R  et al. Proteomic analysis of cells in the early stages of herpes simplex virus type-1 infection reveals widespread changes in the host cell proteome. Proteomics 9:3913-27 (2009). Read more (PubMed: 19670248) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"