製品の概要

  • 製品名Anti-Kettin antibody [MAC 155]
  • 製品の詳細
    Rat monoclonal [MAC 155] to Kettin
  • 特異性Antibody reacts with 500 and 700 kDa isoforms in Lethocerus and Drosophila flight and non-flight muscle. The epitope is near the middle of the molecule. Antibody reacts with Caenorhabditis (nematode) muscle and does not react with rabbit skeletal muscle.
  • アプリケーション適用あり: ICC/IF, Electron Microscopy, WBmore details
  • 種交差性
    交差種: Caenorhabditis elegans, Drosophila melanogaster, Lethocerus indicus (Waterbug)
    非交差種: Rabbit
  • 免疫原

    Z-discs purified from Lethocerus indicus (Waterbug)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
Electron Microscopy Use at an assay dependent dilution.
WB Use at an assay dependent concentration. PubMed: 22479211

ターゲット情報

  • 機能Key component in the assembly and functioning of adult and embryonic striated muscles and muscle tendons. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.
  • 組織特異性Expressed in the mesoderm at stage 11, several hours before myoblast fusion, and persists in most muscle cells, somatic, visceral and pharyngeal muscles and their precursors, until the third instar. Also expressed in adult flight muscle Z-disks.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 7 fibronectin type-III domains.
    Contains 53 Ig-like (immunoglobulin-like) domains.
    Contains 1 SH3 domain.
    Contains 7 TPR repeats.
  • 発生段階Expressed both maternally and zygotically.
  • 細胞内局在Cytoplasm. Nucleus. Chromosome. Uniformly distributed along condensed mitotic chromosomes.
  • Information by UniProt
  • 参照データベース
    • 別名
      • D Titin antibody
      • D-Titin antibody
      • Kettin antibody
      • Sallimus antibody
      • sls antibody
      • Titin antibody
      • TITIN_DROME antibody
      see all

    Anti-Kettin antibody [MAC 155] 画像

    • Immunofluorescence analysis of Drosophila melanogaster larvae muscle, staining Kettin with ab50585.

    Anti-Kettin antibody [MAC 155] (ab50585) 使用論文

    This product has been referenced in:
    • Wójtowicz I  et al. Drosophila small heat shock protein CryAB ensures structural integrity of developing muscles, and proper muscle and heart performance. Development 142:994-1005 (2015). Read more (PubMed: 25715399) »
    • Molnár I  et al. DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila. PLoS Genet 10:e1004166 (2014). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 24586196) »

    See all 10 Publications for this product

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