製品の概要

  • 製品名Anti-Ken and Barbie antibody
  • 製品の詳細
    Mouse polyclonal to Ken and Barbie
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Fusion protein: WKTTDSAYLS SKCGGLRDRA DRRKQQYTSP QNLEPDQTLK YEVDSVDESR NAADFSSAFN SNDNCESAAE CERSGGHNNK EEDEDDCTHK DNKSDKDTDE, corresponding to amino acids 121/220 of Fruit fly (Drosophila melanogaster) Ken and Barbie

  • 特記事項

    Produced from outbred CD1 mice.

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファーConstituents: 50% Glycerol
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab21780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 67 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性Mutations in the ken and barbie locus are accompanied by the malformation of terminalia in adult Drosophila. Male and female genitalia often remain inside the body, and the same portions of genitalia and analia are missing in a fraction of homozygous flies. Rotated and/or duplicated terminalia are also observed. Terminalia phenotypes are enhanced by mutations in the gap gene tailless, the homeobox gene caudal, and the decapentaplegic gene that encodes a TGFbeta-like morphogen. The ken and barbie gene encodes a protein with three CCHH-type zinc finger motifs that are conserved in several transcription factors such as Kruppel and BCL-6. All defects in ken and barbie mutants are fully rescued by the expression of a wild-type genomic construct, which establishes the causality between phenotypes and the gene. Defects in ken result in low mating success and reduced copulation duration. Male and female genitalia often remain inside the body, and genitalia and analia are missing in some homozygous flies. These flies are named after the famous dolls who also lack these 'features'.
  • 細胞内局在Nuclear
  • 参照データベース
    • 別名
      • Ken & Barbie antibody
      • ken and barbie antibody
      • ken-and-barbie antibody
      • Probable transcription factor Ken antibody

    Anti-Ken and Barbie antibody (ab21780) 使用論文

    This product has been referenced in:
    • Kugler SJ  et al. The Putzig-NURF nucleosome remodeling complex is required for ecdysone receptor signaling and innate immunity in Drosophila melanogaster. Genetics 188:127-39 (2011). Read more (PubMed: 21385730) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application IHC - Wholemount
    Sample Fruit fly (Drosophila melanogaster) Embryo (wholemount)
    Specification wholemount
    Username

    Abcam user community

    Verified customer

    投稿 Jul 31 2013



    You are correct that we do have a testing discount scheme called the Abtrial. This offer means that if you purchase the three antibodies you are interested in as normal, test the antibody with your Drosophila samples in IHC and let us know of...

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    Thank you very much for your interest in ab21780. To our knowledge, ab21780 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab21780 now, test it in ICC/IF and submit feedback to us in the form of an Abr...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"