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Synthetic peptide: ETWTFVAPMK HRRSALGITV HQGRIYVLGG YDGHTFLDSV ECYDPDTDTW , corresponding to amino acids 542-591 of Human Keap1
Our Abpromise guarantee covers the use of ab66620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).Can be blocked with Human Keap1 peptide (ab111654). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
This image is a courtesy of Anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Keap1 with ab66620 at a concentration of 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule. Magnification: 400X.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"