製品の概要

  • 製品名Anti-Keap1 antibody
    Keap1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Keap1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide: ETWTFVAPMK HRRSALGITV HQGRIYVLGG YDGHTFLDSV ECYDPDTDTW , corresponding to amino acids 542-591 of Human Keap1

  • ポジティブ・コントロール
    • HepG2 cell lysate. Human kidney tissue.
  • 特記事項Expression of Keap1 shuttles between cytoplasm and nucleus.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).Can be blocked with Human Keap1 peptide (ab111654). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
  • 組織特異性Broadly expressed, with highest levels in skeletal muscle.
  • 配列類似性Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • ドメインThe Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
  • 翻訳後修飾Ubiquitinated and subject to proteasomal degradation.
  • 細胞内局在Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cytosolic inhibitor of Nrf2 antibody
    • INrf 2 antibody
    • INrf2 antibody
    • Keap 1 antibody
    • KEAP1 antibody
    • KEAP1_HUMAN antibody
    • Kelch like ECH associated protein 1 antibody
    • Kelch like family member 19 antibody
    • Kelch like protein 19 antibody
    • Kelch-like ECH-associated protein 1 antibody
    • Kelch-like protein 19 antibody
    • KIAA0132 antibody
    • KLHL 19 antibody
    • KLHL19 antibody
    • MGC10630 antibody
    • MGC1114 antibody
    • MGC20887 antibody
    • MGC4407 antibody
    • MGC9454 antibody
    see all

Anti-Keap1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Keap1 with ab66620 at a concentration of 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule. Magnification: 400X.

  • ICC/IF image of ab66620 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Lane 1 : Anti-Keap1 antibody (ab66620) at 5 µg/ml
    Lane 2 : Anti-Keap1 antibody (ab66620) at 2.5 µg/ml
    Lane 3 : Anti-Keap1 antibody (ab66620) at 1.25 µg/ml

    Lane 1 : Cell lysate prepared form HepG2 cells
    Lane 2 : Cell lysate prepared form HepG2 cells
    Lane 3 : Cell lysate prepared form HepG2 cells

    Lysates/proteins at 25 µg/ml per lane.


    Predicted band size : 70 kDa
  • All lanes : Anti-Keap1 antibody (ab66620) at 1.25 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 70 kDa
  • Anti-Keap1 antibody (ab66620) at 1 µg/ml + Lysate prepared from human 293T cells at 10 µg

    Secondary
    IRDye® 700DX-conjugated donkey polyclonal to rabbit IgG at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This image is a courtesy of Anonymous Abreview

    See Abreview

Anti-Keap1 antibody (ab66620) 使用論文

This product has been referenced in:
  • Orthwein A  et al. A mechanism for the suppression of homologous recombination in G1 cells. Nature 528:422-6 (2015). Read more (PubMed: 26649820) »
  • Baqader NO  et al. Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress. J Proteome Res 13:4398-423 (2014). WB ; Human . Read more (PubMed: 25133973) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Dog Tissue lysate - other (brain)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 20 µg
Specification brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
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投稿 Aug 19 2016

Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Feb 29 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Feb 29 2016

Application Western blot
Sample Mouse Tissue lysate - nuclear (liver nuclear fraction)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 35 µg
Treatment hyperoxia for 44 hours
Specification liver nuclear fraction
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Nov 19 2015

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (Jurkat cell lysate)
Specification Jurkat cell lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Jun 02 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Jun 02 2014

Gracias por contactarnos de nuevo.

El anticuerpo Anti-Caveolin 1 (ab18199) ha sido testado en inmunoprecipitación, Western Blot e immunohistoquímica en secciones embebidas en parafina. Al haberse validado en estas aplicaciones e...

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Gracias por contactarnos.

El producto ab66620 tiene un precio de €380.00 y actualmente en nuestra oficina de Inglaterra queda una unidad es stock. Los productos ab31163 y ab18199 valen €410.00 cada uno y tenemos suficientes unidades...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Loading amount 20 µg
Specification THP-1
Gel Running Conditions Reduced Denaturing (5-20% gradient)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Jan 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 10 µg
Specification 293T
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"