Anti-KDM6B / JMJD3 抗体 - ChIP Grade (ab85392)

製品の概要

  • 製品名Anti-KDM6B / JMJD3 antibody - ChIP Grade
    KDM6B / JMJD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KDM6B / JMJD3 - ChIP Grade
  • アプリケーション適用あり: CLIP, ICC/IF, ChIP, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse KDM6B/ JMJD3 aa 600-650.
    Database link: Q5NCY0

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
CLIP Use at an assay dependent concentration. PubMed: 22589717
ICC/IF 1/200.
ChIP Use at an assay dependent concentration. PubMed: 21095589
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 180 kDa.

ターゲット情報

  • 機能Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
  • 配列類似性Belongs to the UTX family.
    Contains 1 JmjC domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Histone demethylase JMJD3 antibody
    • JmjC domain containing protein 3 antibody
    • JmjC domain-containing protein 3 antibody
    • Jumonji D3 antibody
    • Jumonji domain containing 3 antibody
    • Jumonji domain containing 3 histone lysine demethylase antibody
    • Jumonji domain containing protein 3 antibody
    • Jumonji domain-containing protein 3 antibody
    • Kdm6b antibody
    • KDM6B_HUMAN antibody
    • KIAA0346 antibody
    • Lysine demethylase 6B antibody
    • Lysine K specific demethylase 6B antibody
    • Lysine specific demethylase 6B antibody
    • Lysine-specific demethylase 6B antibody
    see all

Anti-KDM6B / JMJD3 antibody - ChIP Grade 画像

  • Anti-KDM6B / JMJD3 antibody - ChIP Grade (ab85392) at 2 µg/ml + HepG2 cell lysate

    Predicted band size : 180 kDa
    Observed band size : 180 kDa
    Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemistry/Immunofluorescence: ab85392 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with ab85392 at a 1/200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Anti-KDM6B / JMJD3 antibody - ChIP Grade (ab85392) 使用論文

This product has been referenced in:
  • Iida A  et al. Histone demethylase Jmjd3 is required for the development of subsets of retinal bipolar cells. Proc Natl Acad Sci U S A 111:3751-6 (2014). ChIP . Read more (PubMed: 24572572) »
  • Shi X  et al. An epigenetic switch induced by Shh signalling regulates gene activation during development and medulloblastoma growth. Nat Commun 5:5425 (2014). ChIP ; Mouse . Read more (PubMed: 25370275) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (Gradient (4-30%))
Sample Rat Tissue lysate - whole (Brain homogenate)
Specification Brain homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Jan 09 2014

Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 30 µg
Specification HCT116
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 18 2012

Thank you for submitting an Abreview with us.
I am sorry to hear you have been experiencing problems with this ab85392. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints. Read More

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I am very pleased to hear you would like to accept our offer and test ab85392 in chicken. This code will give you xxx off your next order before the expir...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Non-Denaturing (Native)
Sample Chicken Cell lysate - whole cell (brain)
Specification brain
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Ms. Ying Jiang

Verified customer

投稿 Oct 09 2012

Thank you for contacting us.

Abcam uses a service to comb publications for references to our products and add them to the appropriate datasheets. In this particular case I too was wondering how this associated with ab85392. However after f...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

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Thank you for the additional details!
Your protocol seems fine and this protein should typically be expressed in tumor cells so I think the protein should be there to ChIP. As this vial of ab85392 is not working as expected, I am happy to offer a ...

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