Anti-KDM6B / JMJD3 抗体 (ab84200)

製品の概要

  • 製品名Anti-KDM6B / JMJD3 antibody
    KDM6B / JMJD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KDM6B / JMJD3
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human KDM6B/ JMJD3 aa 2-51 (N terminal).
    Sequence:

    HRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASI


    Database link: O15054

  • ポジティブ・コントロール
    • Human fetal lung lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 181 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
  • 配列類似性Belongs to the UTX family.
    Contains 1 JmjC domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Histone demethylase JMJD3 antibody
    • JmjC domain containing protein 3 antibody
    • JmjC domain-containing protein 3 antibody
    • Jumonji D3 antibody
    • Jumonji domain containing 3 antibody
    • Jumonji domain containing 3 histone lysine demethylase antibody
    • Jumonji domain containing protein 3 antibody
    • Jumonji domain-containing protein 3 antibody
    • Kdm6b antibody
    • KDM6B_HUMAN antibody
    • KIAA0346 antibody
    • Lysine demethylase 6B antibody
    • Lysine K specific demethylase 6B antibody
    • Lysine specific demethylase 6B antibody
    • Lysine-specific demethylase 6B antibody
    see all

Anti-KDM6B / JMJD3 antibody 画像

  • Anti-KDM6B / JMJD3 antibody (ab84200) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 181 kDa
    Observed band size : >168 kDa (why is the actual band size different from the predicted?)

Anti-KDM6B / JMJD3 antibody (ab84200) 使用論文

ab84200 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (5-15%)
Sample Mouse Cell lysate - whole cell (Human Fibroblasts Mouse Primary hippocampal neuron)
Specification Human Fibroblasts Mouse Primary hippocampal neuron
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Sep 27 2013

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Thank you for your reply.
I am sorry to hear that you are struggeling so much with these antibodies.

I have checked again the datasheet for ab85392: Mouse was shown to work in the publication that is listed on the datasheet:

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I am sorry to hear that the KDM6B antibodies do not work for you satisfactorily.

According to our correspondence, your are working on mouse samples and only ab85392 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"