製品の概要

  • 製品名
    Anti-KDM6B / JMJD3 antibody
    KDM6B / JMJD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KDM6B / JMJD3
  • アプリケーション
    適用あり: IHC (PFA fixed), ICC/IF, ChIPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human KDM6B/ JMJD3 aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: O15054
    (Peptide available as ab38112)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC (PFA fixed) Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 23932971

ターゲット情報

  • 機能
    Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
  • 配列類似性
    Belongs to the UTX family.
    Contains 1 JmjC domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Histone demethylase JMJD3 antibody
    • JmjC domain containing protein 3 antibody
    • JmjC domain-containing protein 3 antibody
    • Jumonji D3 antibody
    • Jumonji domain containing 3 antibody
    • Jumonji domain containing 3 histone lysine demethylase antibody
    • Jumonji domain containing protein 3 antibody
    • Jumonji domain-containing protein 3 antibody
    • Kdm6b antibody
    • KDM6B_HUMAN antibody
    • KIAA0346 antibody
    • Lysine demethylase 6B antibody
    • Lysine K specific demethylase 6B antibody
    • Lysine specific demethylase 6B antibody
    • Lysine-specific demethylase 6B antibody
    see all

Anti-KDM6B / JMJD3 antibody 画像

  • Chromatin was prepared from mouse hippocampus. Crosslinking (X-ChIP) was performed for 15 minutes with 1% formaldehyde. ChIP was performed with ab38113 at 1/50 dilution (black) or normal rabbit IgG (white). The positive control was Beta-actin. The negative control was HoxA1 (a polycomb repressed gene in hippocampus).

    See Abreview

  • ICC/IF image of ab38113 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab38113, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, MCF7 and HepG2 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
  • IHC image of KDM6B/JMJD3 staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38113, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-KDM6B / JMJD3 antibody (ab38113) 使用論文

This product has been referenced in:
  • Xun J  et al. JMJD3 suppresses stem cell-like characteristics in breast cancer cells by downregulation of Oct4 independently of its demethylase activity. Oncotarget 8:21918-21929 (2017). WB ; Human . Read more (PubMed: 28423536) »
  • Gan X  et al. Epigenetically repressing human cytomegalovirus lytic infection and reactivation from latency in THP-1 model by targeting H3K9 and H3K27 histone demethylases. PLoS One 12:e0175390 (2017). Read more (PubMed: 28407004) »

See all 10 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (L929)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
L929
Blocking step
Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 18°C
Username

Abcam user community

Verified customer

投稿 Aug 30 2017

Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Tissue lysate - nuclear (Hippocampus)
Specification
Hippocampus
Negative control
HoxA1, a Polycomb repressed gene in hippocampus, and normal Rabbit IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehide 1%
Positive control
b-actin
Username

Dr. Ernest Palomer Vila

Verified customer

投稿 Nov 11 2014

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing
Sample
Chicken Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Ying Jiang

Verified customer

投稿 Aug 07 2013

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your reply and for confirming the steps of your protocol.


Since these antibodies are Fast Track antibodies, they are only guaranteed to work in ELISA using the peptide immunogen. These are not tested in-house, and therefor...

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, HEK293, Jurkat, HL-60)
Loading amount
20 µg
Specification
HeLa, HEK293, Jurkat, HL-60
Gel Running Conditions
Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Feb 22 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Mar 16 2009

Application
Western blot
Sample
Human Cell lysate - whole cell (keratinocyte)
Loading amount
100 µg
Specification
keratinocyte
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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