This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

ターゲット情報

  • 機能Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.
  • 配列類似性Belongs to the UTX family.
    Contains 1 JmjC domain.
    Contains 8 TPR repeats.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX) antibody
    • bA386N14.2 antibody
    • bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX antibody
    • DKFZp686A03225 antibody
    • Histone demethylase UTX antibody
    • KABUK2 antibody
    • Kdm6a antibody
    • KDM6A_HUMAN antibody
    • Lysine (K) specific demethylase 6A antibody
    • Lysine K specific demethylase 6A antibody
    • Lysine specific demethylase 6A ubiquitously transcribed tetratricopeptide repeat gene X chromosome antibody
    • Lysine-specific demethylase 6A antibody
    • MGC141941 antibody
    • Ubiquitously transcribed tetratricopeptide repeat X chromosome antibody
    • Ubiquitously transcribed tetratricopeptide repeat protein X linked antibody
    • Ubiquitously transcribed TPR gene on the X chromosome antibody
    • Ubiquitously transcribed TPR protein on the X chromosome antibody
    • Ubiquitously transcribed X chromosome tetratricopeptide repeat protein antibody
    • Ubiquitously-transcribed TPR protein on the X chromosome antibody
    • Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein antibody
    • UTX antibody
    see all

Anti-KDM6A / UTX antibody 画像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-KDM6A / UTX antibody (ab36938) at 1 µg/ml

    Lane 1 : Whole NIH3T3 cell lysate (no shRNA)
    Lane 2 : Whole NIH3T3 cell lysate (Control shRNA)
    Lane 3 : Whole NIH3T3 cell lysate (Utx shRNA)

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit polyclonal
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 154 kDa
    Observed band size : 148 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    Lane 3 is NIH3T3 cells transduced with lentivirus expressing shRNA for mouse Utx.

    5% milk was used as the blocking agent (incubated for 1 hour at 25°C).

    ab36938 was diluted with TBS+0.1% Tween 20 and incubated for 1 hour at 25°C. 

    See Abreview

  • All lanes : Anti-KDM6A / UTX antibody (ab36938) at 1 µg/ml

    Lane 1 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG -H&L- Pre Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 154 kDa
    Observed band size : 154,157 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
    This Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
  • ICC/IF image of ab36938 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab36938, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa cells fixed in 4% PFA at 1ug/ml and HeLa and MCF7 100% methanol fixed cells at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

Anti-KDM6A / UTX antibody (ab36938) 使用論文

This product has been referenced in:
  • Li Q  et al. Stage-dependent and locus-specific role of histone demethylase Jumonji D3 (JMJD3) in the embryonic stages of lung development. PLoS Genet 10:e1004524 (2014). WB . Read more (PubMed: 25079229) »
  • Ntziachristos P  et al. Contrasting roles of histone 3 lysine 27 demethylases in acute lymphoblastic leukaemia. Nature N/A:N/A (2014). Read more (PubMed: 25132549) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (7,5 %, tank blot to PVDF)
Sample Human Cell lysate - whole cell (U2OS cell line)
Specification U2OS cell line
Treatment -/+ transfection with full length UTX expression plasmid
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 20 2013

Thank you for your reply and for confirming the steps of your protocol.


Since these antibodies are Fast Track antibodies, they are only guaranteed to work in ELISA using the peptide immunogen. These are not tested in-house, and therefor...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain Section)
Specification Brain Section
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization Yes - 0.1% Tween20
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Oct 08 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (Heart, Liver and Kidney)
Loading amount 25 µg
Specification Heart, Liver and Kidney
Gel Running Conditions Reduced Denaturing (NuPAGE BisTris 10% gel (invitrogen))
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: 22°C
Username

Dr. Vanessa Hall

Verified customer

投稿 Aug 20 2010

Application Western blot
Sample Mouse Cell lysate - nuclear (NIH3T3 cell lysate)
Loading amount 20 µg
Specification NIH3T3 cell lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"