KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)

製品の概要

  • 製品名
    KDM5/JARID Activity Quantification Assay Kit (Fluorometric)
    KDM5/JARID キット 製品一覧
  • アッセイタイプ
    Enzyme activity
  • 全工程の試験時間
    3h 00m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Mammal
  • 製品の概要

    KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.

    KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464) is designed for the easy and fast fluorometric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

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  • Demonstration of high sensitivity of KDM5/JARID activity assay achieved by using A549 nuclear extracts with ab113464

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参考文献

ab113464 has not yet been referenced specifically in any publications.

レビューと Q&A

Ce kit est pour mesurer l’activité de KDM5 qui est une enzyme qui enlève les methyl des protéines et surtout des histones.

KDM5 a effectivement comme substrats, H3K4me3 et H3K4me2 et produit H3K4 mono-méthyl&e...

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Thank you for your interest in ab113455 & ab113464.

You are correct that both these kits measure demethylase activity. The major difference between these 2 kits is the substrate captured on the well. For ab113455, the substrate is a dimet...

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Thank you for your call last week and for your patience while I have been in touch with my colleagues in the lab regarding ab113464.

The substrate is a short peptide, and the detection antibody binds to mono-methylated histone.

I ho...

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Thank you for contacting us. Tri-methylated histone H3-K4 is a substrate for JARID enzyme which removes methyl group from substrate, which is then detected by antibodies present in the kit. So the kit antibodies will detect the JARID de-methyla...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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