Anti-KDM5B / PLU1 / Jarid1B 抗体 (ab56759)

製品の概要

  • 製品名Anti-KDM5B / PLU1 / Jarid1B antibody
    KDM5B / PLU1 / Jarid1B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to KDM5B / PLU1 / Jarid1B
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: AKRMRAEAMN IKIEPEETTE ARTHNLRRRM GCPTPKCENE KEMKSSIKQE PIERKDYIVE NEKEKPKSRS KKATNAVDLY VCLLCGSGN, corresponding to amino acids 231-320 of Human PLU1/ Jarid1B

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 190 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
  • 組織特異性Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).
  • 配列類似性Belongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 3 PHD-type zinc fingers.
  • ドメインBoth the JmjC domain and the JmjN domain are required for enzymatic activity.
    The 2 first PHD-type zinc finger domains are required for transcription repression activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancer/testis antigen 31 antibody
    • CT31 antibody
    • Histone demethylase JARID1B antibody
    • JARID1B antibody
    • Jumonji AT rich interactive domain 1B antibody
    • Jumonji/ARID domain-containing protein 1B antibody
    • Kdm5b antibody
    • KDM5B_HUMAN antibody
    • Lysine (K) specific demethylase 5B antibody
    • Lysine demethylase 5B antibody
    • Lysine specific demethylase 5B antibody
    • Lysine-specific demethylase 5B antibody
    • PLU-1 antibody
    • PLU1 antibody
    • PPP1R98 antibody
    • Protein phosphatase 1 regulatory subunit 98 antibody
    • PUT1 antibody
    • putative DNA/chromatin binding motif antibody
    • RBBP2H1A antibody
    • RBP2 like antibody
    • RBP2-H1 antibody
    • Retinoblastoma-binding protein 2 homolog 1 antibody
    • Retinoblastoma-binding protein 2 homolog 1A antibody
    see all

Anti-KDM5B / PLU1 / Jarid1B antibody 画像

  • PLU1 / Jarid1B antibody (ab56759) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.


  • Predicted band size : 190 kDa
  • All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody (ab56759) at 1/500 dilution

    Lane 1 : Human Pancreatic Cancer whole cell lysate (Line AsPc1)
    Lane 2 : Human Pancreatic Cancer whole cell lysate (line Panc1)
    Lane 3 : Human Pancreatic Cancer whole cell lysate (Line CaPan1)

    Lysates/proteins at 35 µg per lane.

    Secondary
    An HRP-conjugated Sheep anti-mouse polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 190 kDa
    Observed band size : 190 kDa


    Exposure time : 15 minutes

    This image is courtesy of an Abreview submitted by Pawel Mazur

    See Abreview

  • ab56759 staining KDM5B/PLU1/Jarid1B in Human pancreatic cancer tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with PBS + Triton 0.025% and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab56759 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26759, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing MCF7 cells stained with ab56759 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56759, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-KDM5B / PLU1 / Jarid1B antibody (ab56759) 使用論文

ab56759 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% SDS)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Abcam user community

Verified customer

投稿 Apr 18 2014

Thank you for contacting us.

I am sorry to hear you have been experiencing problems with ab50598. I have contacted the lab to obtain images for bothab45301 and ab50958. Could you confirm whether ab56759 worked successfully? I would also like...

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Thank you for your enquiry regarding ab56759 and ab85454; and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

I would recommend the following posi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human pancreatic cancer)
Specification Human pancreatic cancer
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization Yes - PBS + Triton 0.025%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jul 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (pancreatic cancer)
Loading amount 35 µg
Specification pancreatic cancer
Gel Running Conditions Non-reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Pawel Mazur

Verified customer

投稿 Jun 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"