Anti-KDM5A / Jarid1A / RBBP2 抗体 - ChIP Grade (ab70892)

製品の概要

  • 製品名
    Anti-KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade
    KDM5A / Jarid1A / RBBP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KDM5A / Jarid1A / RBBP2 - ChIP Grade
  • アプリケーション
    適用あり: WB, IP, ICC/IF, ChIPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human KDM5A/ Jarid1A/ RBBP2 (C terminal).
    Database link: P29375

  • ポジティブ・コントロール
    • HeLa, 293T and NIH3T3 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 196 kDa (predicted molecular weight: 196 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12.
  • 配列類似性
    Belongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 3 PHD-type zinc fingers.
  • ドメイン
    The GSGFP motif is required for the interaction with SUZ12.
  • 細胞内局在
    Nucleus > nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Histone demethylase JARID1A antibody
    • JARID1A antibody
    • Jumonji/ARID domain containing protein 1A antibody
    • Jumonji/ARID domain-containing protein 1A antibody
    • Kdm5a antibody
    • KDM5A_HUMAN antibody
    • Lysine-specific demethylase 5A antibody
    • RBBP-2 antibody
    • RBBP2 antibody
    • RBP2 antibody
    • Retinoblastoma binding protein 2 antibody
    • Retinoblastoma-binding protein 2 antibody
    see all

画像

  • All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade (ab70892) at 1/2000 dilution

    Lane 1 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
    Lane 2 : Renca cell lysate


    Predicted band size : 196 kDa
  • PLA assay using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

  • All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade (ab70892) at 1/2000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate


    Predicted band size : 196 kDa
  • All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade (ab70892) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 196 kDa
    Observed band size : 196 kDa


    Exposure time : 3 minutes
  • ICC/IF image of ab70892 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70892, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70892 at 1µg/ml for WB, after IP at 3 µg/mg lysate.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

参考文献

This product has been referenced in:
  • Brier AB  et al. The KDM5 family is required for activation of pro-proliferative cell cycle genes during adipocyte differentiation. Nucleic Acids Res 45:1743-1759 (2017). WB, ChIP ; Mouse . Read more (PubMed: 27899593) »
  • Qiu MT  et al. KDM4B and KDM4A promote endometrial cancer progression by regulating androgen receptor, c-myc, and p27kip1. Oncotarget 6:31702-20 (2015). ChIP . Read more (PubMed: 26397136) »

See all 5 Publications for this product

レビューと Q&A

Application
Immunoprecipitation
Immuno-precipitation step
Other - Protein G dynabeads
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Total protein in input
300 µg
Username

Dr. Edyta Marcon

Verified customer

投稿 Apr 01 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 10 2014

J’ai contacté le laboratoire qui a confirmé que l’immunogène d’ab70892 correspond à une région entre les acides aminés 1640 et 1690 de la protéine humaine KDM5A / Jarid1A / RBBP2, NP_00...

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Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (10 % GEL, SDS)
Loading amount
20 µg
Treatment
siRNA transfection
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Apr 18 2014

DISCOUNT CODE: ABTRIAL-XXXXX
Expiration date: 02/04/2013
Value: :€370.00

I am very pleased to hear you would like to accept our offer and test ab70892 for ChIP-sequencing. This code will give you €370.00 off your next order b...

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Thank you for your enquiry. I am sorry that the product you were using is out of our catalogue.

We would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications liste...

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Thank you for contacting us. Your credit note ID is xxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - whole cell (pancreatic ilset tumor cells)
Specification
pancreatic ilset tumor cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Real-time PCR
Positive control
Cops7b locus
Pcid2 locus
Negative control
Gapdh3UTR
Gata63UTR
Username

Abcam user community

Verified customer

投稿 May 02 2012

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab70892.

To check the status of the order please contact o...

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Thank you for contacting us and your interest in our products. Currently we have two lots of Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) in stock. These are both of lot number GR70517-1. I hope this information has been of help. If your customernee...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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