Anti-KDM5A / Jarid1A / RBBP2 抗体 (ab70892)
Key features and details
- Rabbit polyclonal to KDM5A / Jarid1A / RBBP2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KDM5A / Jarid1A / RBBP2 antibody
KDM5A / Jarid1A / RBBP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KDM5A / Jarid1A / RBBP2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to Human KDM5A/ Jarid1A/ RBBP2 (C terminal).
Database link: P29375 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70892の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (3) |
1/2000 - 1/10000. Detects a band of approximately 196 kDa (predicted molecular weight: 196 kDa).
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IP | (1) |
Use a concentration of 2 - 5 µg/ml.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 196 kDa (predicted molecular weight: 196 kDa). |
IP
Use a concentration of 2 - 5 µg/ml. |
ターゲット情報
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機能
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. -
配列類似性
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers. -
ドメイン
The GSGFP motif is required for the interaction with SUZ12. -
細胞内局在
Nucleus > nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function. - Information by UniProt
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参照データベース
- Entrez Gene: 5927 Human
- Entrez Gene: 214899 Mouse
- Entrez Gene: 312678 Rat
- Omim: 180202 Human
- SwissProt: P29375 Human
- SwissProt: Q3UXZ9 Mouse
- Unigene: 76272 Human
- Unigene: 404761 Mouse
see all -
別名
- Histone demethylase JARID1A antibody
- JARID1A antibody
- Jumonji/ARID domain containing protein 1A antibody
see all
画像
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All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) at 1/2000 dilution
Lane 1 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 2 : Renca cell lysate
Predicted band size: 196 kDa -
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70892 at 1µg/ml for WB, after IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 196 kDa -
All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 196 kDa
Observed band size: 196 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab70892 は 21 報の論文で使用されています。
- Domingo-Reinés J et al. Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus. Front Cell Dev Biol 10:846092 (2022). PubMed: 35721502
- Li S et al. Fbxo22 inhibits metastasis in triple-negative breast cancer through ubiquitin modification of KDM5A and regulation of H3K4me3 demethylation. Cell Biol Toxicol N/A:N/A (2022). PubMed: 36112263
- Ma YS et al. KDM5A silencing transcriptionally suppresses the FXYD3-PI3K/AKT axis to inhibit angiogenesis in hepatocellular cancer via miR-433 up-regulation. J Cell Mol Med 25:4040-4052 (2021). PubMed: 33621431
- Zhou M et al. miR-181d/RBP2/NF-?B p65 Feedback Regulation Promotes Chronic Myeloid Leukemia Blast Crisis. Front Oncol 11:654411 (2021). PubMed: 33842368
- Karia D et al. The histone H3K4 demethylase JARID1A directly interacts with haematopoietic transcription factor GATA1 in erythroid cells through its second PHD domain. R Soc Open Sci 7:191048 (2020). PubMed: 32218938