Anti-KDM4D / JMJD2D 抗体 (ab93694)
Key features and details
- Rabbit polyclonal to KDM4D / JMJD2D
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-KDM4D / JMJD2D antibody
KDM4D / JMJD2D 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KDM4D / JMJD2D -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 1-354 of Human KDM4D/ JMJD2D (43kDa).
-
特記事項
The immunogen used for this product shares 64%, 71% and 65% homology with KDM4A/JMJD2A, KDM4B/JMJD2B and KDM4C/JMJD2C respectively. Cross-reactivity with these proteins has not been confirmed experimentally.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.05% BSA, 50% Glycerol, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93694の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/200. Predicted molecular weight: 58 kDa.
|
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
特記事項 |
---|
WB
1/200. Predicted molecular weight: 58 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
-
機能
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. -
配列類似性
Belongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 55693 Human
- Omim: 609766 Human
- SwissProt: Q6B0I6 Human
- Unigene: 503598 Human
-
別名
- JHD3D antibody
- JHDM3D antibody
- JmjC domain-containing histone demethylation protein 3D antibody
see all
画像
-
All lanes : Anti-KDM4D / JMJD2D antibody (ab93694) at 1/200 dilution
Lane 1 : DLD1 cell lysate at 10 µg
Lane 2 : DLD1 cell lysate at 5 µg
Predicted band size: 58 kDa -
ab93694, at 10µg/ml, staining KDM4D / JMJD2D in DLD1 (Human Colorectal Adenocarcinoma) cells by Immunofluorescence. Cells were fixed with 1% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with normal goat serum. Secondary antibody: Goat anti Rabbit Cy3 at 1/250 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (17)
ab93694 は 17 報の論文で使用されています。
- Liu D et al. TRIM14 inhibits OPTN-mediated autophagic degradation of KDM4D to epigenetically regulate inflammation. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35145029
- Deng Y et al. Histone demethylase JMJD2D promotes the self-renewal of liver cancer stem-like cells by enhancing EpCAM and Sox9 expression. J Biol Chem 296:100121 (2021). PubMed: 33434575
- Yan H et al. Histone demethylase KDM4D inhibition suppresses renal cancer progression and angiogenesis through JAG1 signaling. Cell Death Discov 7:284 (2021). PubMed: 34667158
- Li M et al. Demethylase-independent function of JMJD2D as a novel antagonist of p53 to promote Liver Cancer initiation and progression. Theranostics 10:8863-8879 (2020). PubMed: 32754284
- Dukaew N et al. Enhancement of Radiosensitivity by Eurycomalactone in Human NSCLC Cells Through G2/M Cell Cycle Arrest and Delayed DNA Double-Strand Break Repair. Oncol Res 28:161-175 (2020). PubMed: 31727206