製品の概要

  • 製品名
    Anti-KDM4D / JMJD2D antibody
    KDM4D / JMJD2D 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KDM4D / JMJD2D
  • アプリケーション
    適用あり: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-354 of Human KDM4D/ JMJD2D (43kDa).

  • ポジティブ・コントロール
    • DLD1 (Human colorectal adenocarcinoma) cells; DLD1 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200. Predicted molecular weight: 58 kDa.
IP Use at 20 µg/mg of lysate.
Flow Cyt Use a concentration of 5 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
  • 配列類似性
    Belongs to the JHDM3 histone demethylase family.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • JHD3D antibody
    • JHDM3D antibody
    • JmjC domain-containing histone demethylation protein 3D antibody
    • Jumonji domain-containing protein 2D antibody
    • Kdm4d antibody
    • KDM4D_HUMAN antibody
    • Lysine-specific demethylase 4D antibody
    see all

画像

  • All lanes : Anti-KDM4D / JMJD2D antibody (ab93694) at 1/200 dilution

    Lane 1 : DLD1 cell lysate at 10 µg
    Lane 2 : DLD1 cell lysate at 5 µg


    Predicted band size : 58 kDa
  • ab93694, at 10µg/ml, staining KDM4D / JMJD2D in DLD1 (Human Colorectal Adenocarcinoma) cells by Immunofluorescence. Cells were fixed with 1% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with normal goat serum. Secondary antibody: Goat anti Rabbit Cy3 at 1/250 dilution.
  • IHC image of ab93694 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93694, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Wu R  et al. H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication. Nucleic Acids Res 45:169-180 (2017). WB, ChIP, ICC/IF . Read more (PubMed: 27679476) »
  • Cleys ER  et al. Androgen receptor and histone lysine demethylases in ovine placenta. PLoS One 10:e0117472 (2015). IP ; Sheep . Read more (PubMed: 25675430) »

See all 8 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab93694.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録