Anti-KDM4C / GASC1 / JMJD2C 抗体 (ab27532)

製品の概要

  • 製品名
    Anti-KDM4C / GASC1 / JMJD2C antibody
    KDM4C / GASC1 / JMJD2C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KDM4C / GASC1 / JMJD2C
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human GASC1/ JMJD2C.

    (Peptide available as ab30652.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Whole Cell Lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line) This antibody gave a positive signal in the following Methanol fixed cell line HeLa.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).

ターゲット情報

  • 機能
    Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
  • 組織特異性
    Overexpressed in several esophageal squamous cell carcinomas (ESCs).
  • 配列類似性
    Belongs to the JHDM3 histone demethylase family.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • ドメイン
    The 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA146B14.1 antibody
    • GASC 1 protein antibody
    • GASC-1 protein antibody
    • GASC1 antibody
    • Gene amplified in squamous cell carcinoma 1 protein antibody
    • JHDM3C antibody
    • JmjC domain containing histone demethylation protein 3C antibody
    • JmjC domain-containing histone demethylation protein 3C antibody
    • JMJD2C antibody
    • Jumonji domain containing 2C antibody
    • Jumonji domain containing protein 2C antibody
    • Jumonji domain-containing protein 2C antibody
    • Kdm4c antibody
    • KDM4C_HUMAN antibody
    • Lysine (K) specific demethylase 4C antibody
    • Lysine demethylase 4C antibody
    • Lysine-specific demethylase 4C antibody
    • TDRD14C antibody
    • Tudor domain containing 14C antibody
    see all

Anti-KDM4C / GASC1 / JMJD2C antibody 画像

  • All lanes : Anti-KDM4C / GASC1 / JMJD2C antibody (ab27532) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 120 kDa
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • ICC/IF image of ab27532 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27532 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

プロトコール

Anti-KDM4C / GASC1 / JMJD2C antibody (ab27532) 使用論文

ab27532 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Mar 21 2017


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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
400 µg
Specification
HeLa
Immuno-precipitation step
Protein A
Username

Mr. Antibody Solutions

Verified customer

投稿 May 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293, HeLa, Jurkat, U2OS)
Loading amount
20 µg
Specification
HEK293, HeLa, Jurkat, U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Apr 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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