Rabbit polyclonal to KDM4B / JMJD2B
Synthetic peptide corresponding to Human KDM4B/ JMJD2B aa 4-31 (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: O94953
K562 cell lysate, Human Testis tissue
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Immunogen affinity purified
This antibody is purified through a protein A column, followed by peptide affinity purification.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/100 - 1/500. Predicted molecular weight: 122 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
Belongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain. Contains 1 JmjN domain. Contains 2 PHD-type zinc fingers. Contains 2 Tudor domains.
The 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
Information by UniProt
JmjC domain-containing histone demethylation protein 3B antibody
Western blot - KDM4B / JMJD2B antibody (ab103129)
Anti-KDM4B / JMJD2B antibody (ab103129) at 1/100 dilution + K562 cell lysate at 35 µg
Predicted band size: 122 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM4B / JMJD2B antibody (ab103129)
Formalin-fixed and paraffin-embedded Human Testis tissue, which was peroxidase-conjugated to the
secondary antibody, followed by DAB staining, using ab103129 at a dilution of 1/50.
Flow Cytometry - Anti-KDM4B / JMJD2B antibody (ab103129)
Flow cytometry analysis of K562 cellls (right histogram) compared to a negative control cell (left histogram) using ab103129.FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.
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