Anti-KDM4A / JHDM3A / JMJD2A 抗体 - ChIP Grade (ab24545)

製品の概要

  • 製品名
    Anti-KDM4A / JHDM3A / JMJD2A antibody - ChIP Grade
    KDM4A / JHDM3A / JMJD2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KDM4A / JHDM3A / JMJD2A - ChIP Grade
  • アプリケーション
    適用あり: ICC/IF, IP, ChIP, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 592-720 of Human JMJD2A

  • ポジティブ・コントロール
    • HeLa cell nuclear lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution.
WB 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).

ターゲット情報

  • 機能
    Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
    Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the JHDM3 histone demethylase family.
    Contains 1 C2HC pre-PHD-type zinc finger.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • ドメイン
    The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue (H3K4me). Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' (H3K4me3) is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are also able to bind trimethylated histone H3 'Lys-9' (H3K9me3), di- and trimethylated H4 'Lys-20' (H4K20me2 and H4K20me3). Has high affinity for H4K20me2, blocking recruitment of proteins such as TP53BP1.
  • 翻訳後修飾
    Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • JHDM3A antibody
    • JmjC domain containing histone demethylation protein 3A antibody
    • JmjC domain-containing histone demethylation protein 3A antibody
    • JMJD2 antibody
    • JMJD2A antibody
    • jumonji C domain containing histone demethylase 3A antibody
    • Jumonji domain containing 2 antibody
    • Jumonji domain containing 2A antibody
    • Jumonji domain containing protein 2A antibody
    • Jumonji domain-containing protein 2A antibody
    • KDM4A antibody
    • KDM4A_HUMAN antibody
    • KIAA0677 antibody
    • Lysine (K) specific demethylase 4A antibody
    • Lysine-specific demethylase 4A antibody
    • TDRD14A antibody
    • Tudor domain containing 14A antibody
    see all

Anti-KDM4A / JHDM3A / JMJD2A antibody - ChIP Grade 画像

  • Anti-KDM4A / JHDM3A / JMJD2A antibody - ChIP Grade (ab24545) at 1/2000 dilution + HeLa nuclear extract

    Predicted band size : 120 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

Anti-KDM4A / JHDM3A / JMJD2A antibody - ChIP Grade (ab24545) 使用論文

This product has been referenced in:
  • Garcia J & Lizcano F KDM4C Activity Modulates Cell Proliferation and Chromosome Segregation in Triple-Negative Breast Cancer. Breast Cancer (Auckl) 10:169-175 (2016). ICC/IF ; Human . Read more (PubMed: 27840577) »
  • Rosales W  et al. Role of Histone Demethylases in Cardiomyocytes Induced to Hypertrophy. Biomed Res Int 2016:2634976 (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27722168) »

See all 9 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Feb 27 2015

I'm sorry to hear that ab24545 did not work for you in western blot.

As you requested, I have set up an Order 1217875 for ab70786 free of charge. The only vial in stock is being transported to the US, and the expected delivery date to you is *...

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Thank you for your response and detailed protocol description.

Did you try lowering the exposure time of the film until the background luminescence was gone? Also, please respond if you would like a replacement vial of the same antibody, a dif...

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Thank you for contacting Abcam.

Based on the image sent for ab24545, there is definitely non-specific binding occurring. In order to block some of the non-specific binding, please attempt the troubleshooting steps listed below, so we can deter...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HL60 and HeLa)
Loading amount
100 µg
Specification
HL60 and HeLa
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 23 2007

Thank you for your enquiry. I am sorry to hear that you have been experiencing problems with ab24545 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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