製品の概要

  • 製品名
    Anti-KDM3B / JMJD1B antibody
    KDM3B / JMJD1B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KDM3B / JMJD1B
  • アプリケーション
    適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • 免疫原

    Synthetic peptide corresponding to Human KDM3B/ JMJD1B (C terminal).
    Database link: Q7LBC6

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates. IHC Controls: Laryngeal squamous cell carcinoma, Ovarian carcinoma, Prostate carcinoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IF 1/50 - 1/250.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 191 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
  • 組織特異性
    Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.
  • 配列類似性
    Belongs to the JHDM2 histone demethylase family.
    Contains 1 JmjC domain.
  • ドメイン
    Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5qNCA antibody
    • C5orf7 antibody
    • JHDM2B antibody
    • JmjC domain containing histone demethylation protein 2B antibody
    • JmjC domain-containing histone demethylation protein 2B antibody
    • jmjd1b antibody
    • Jumonji domain containing 1B antibody
    • Jumonji domain containing protein 1B antibody
    • Jumonji domain-containing protein 1B antibody
    • KDM3B antibody
    • KDM3B_HUMAN antibody
    • KIAA1082 antibody
    • Lysine (K) specific demethylase 3B antibody
    • Lysine-specific demethylase 3B antibody
    • NET22 antibody
    • Nuclear protein 5qNCA antibody
    see all

Anti-KDM3B / JMJD1B antibody 画像

  • All lanes : Anti-KDM3B / JMJD1B antibody (ab70797) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 191 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,30 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KDM3B / JMJD1B with ab70797 at 1/200 (1µg/ml). Detection: DAB.
  • Detection of KDM3B / JMJD1B by Western Blot of Immunoprecipitate.
    ab70797 at 1µg/ml staining KDM3B / JMJD1B in HeLa whole cell lysate immunoprecipitated using ab70797 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-KDM3B / JMJD1B antibody (ab70797) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (6% Gel)
Loading amount
500000 cells
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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