KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)

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製品の概要

  • 製品名
    KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric)
    KDM1 / LSD1 キット 製品一覧
  • アッセイタイプ
    Enzyme activity
  • 全工程の試験時間
    3h 00m
  • 種交差性
    交差種: Mouse, Human, Plants
    交差が予測される動物種: Mammal, Other
  • 製品の概要

    KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.


    KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459) is designed for the easy and fast colorimetric measurement of KDM1/LSD1 activity or inhibition. The antibody-based, immunospecific method directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.


    This kit may cross react with LSD2.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

  • 保存方法
    Please refer to protocols.
  • 内容 48 tests 96 tests
    10X Wash Buffer 1 x 14ml 1 x 28ml
    8-Well Assay Strips (with Frame) 6 units 12 units
    Adhesive Covering Film 1 unit 1 unit
    Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl
    Detection Antibody, 400 µg/mL 1 x 6µl 1 x 12µl
    Developer Solution 1 x 5ml 1 x 10ml
    LSD1 Assay Buffer 1 x 4ml 1 x 8ml
    LSD1 Assay Standard, 50 µg/mL 1 x 10µl 1 x 20µl
    LSD1 Inhibitor Tranylcypromine, 1 mM 1 x 20µl 1 x 40µl
    LSD1 Substrate, 50 µg/mL 1 x 60µl 1 x 120µl
    Stop Solution 1 x 5ml 1 x 10ml
  • 研究分野
  • 関連性
    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2)H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor(ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
  • 別名
    • Amine oxidase (flavin containing) domain 2
    • AOF2
    • BHC110
    • BRAF35-HDAC complex protein BHC110
    • FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
    • Flavin-containing amine oxidase domain-containing protein 2
    • KDM1
    • KIAA0601
    • LSD1
    • Lysine (K) specific demethylase 1
    • Lysine (K) specific demethylase 1A
    • Lysine-specific histone demethylase 1
    • Lysine-specific histone demethylase 1A
    see all
  • 参照データベース

    アプリケーション

    Our Abpromise guarantee covers the use of ab113459 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    画像

    • Functional Studies - KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
      Functional Studies - KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
      Demonstration of inhibitory effects of an KDM1/LSD1 inhibitor detected by ab113459. [LSD1 concentration: 200 ng/well]
    • Functional Studies - KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
      Functional Studies - KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
      Demonstration of high sensitivity of KDM1 / LSD1 activity assay achieved by using recombinant KDM1 / LSD1 with ab113459.

    プロトコール

    参考文献

    ab113459 has not yet been referenced specifically in any publications.

    ab113459 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    2 reviews or Q&A

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    Votes: Highest First

    Inquiry: I'm looking for a demethylase assay kit for testing of immunocomplexes's activity (containing LSD1) on a possible native substrate (ex recombinant histone rather than peptide). Is your kit 'ab 113472' suitable for this? What is the wave of exc...

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    Thank you for contacting us.
    The kit ab113472 is designed to detect DNA demethylase activity and will detect the this activity using a unique methylated DNA substrate. The detection is then colorimetric and can be measured by optical density measu...

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    I am working for Dana Farber Cancer Institute, Boston, MA.Our labis looking for a ELISA kit which can measure the activity of LSD1.For this, we are trying to understand the difference between all the 4 ELISA kits for LSD1(named below) ABcam is providin...

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    Thank you for contacting Abcam. For ab113459 and ab113460, both kits directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format. The main difference between the 2 are that ab113459, has a co...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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