KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.
KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459) is designed for the easy and fast colorimetric measurement of KDM1/LSD1 activity or inhibition. The antibody-based, immunospecific method directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
This kit may cross react with LSD2.
内容 | 48 tests | 96 tests |
---|---|---|
10X Wash Buffer | 1 x 14ml | 1 x 28ml |
8-Well Assay Strips (with Frame) | 6 units | 12 units |
Adhesive Covering Film | 1 unit | 1 unit |
Capture Antibody, 1000 µg/mL | 1 x 5µl | 1 x 10µl |
Detection Antibody, 400 µg/mL | 1 x 6µl | 1 x 12µl |
Developer Solution | 1 x 5ml | 1 x 10ml |
LSD1 Assay Buffer | 1 x 4ml | 1 x 8ml |
LSD1 Assay Standard, 50 µg/mL | 1 x 10µl | 1 x 20µl |
LSD1 Inhibitor Tranylcypromine, 1 mM | 1 x 20µl | 1 x 40µl |
LSD1 Substrate, 50 µg/mL | 1 x 60µl | 1 x 120µl |
Stop Solution | 1 x 5ml | 1 x 10ml |
Our Abpromise guarantee covers the use of ab113459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Functional Studies | Use at an assay dependent concentration. |
ab113459 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"