製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KCTD7
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (VVVTGREPDS RRQDGAMSSS DAEDDFLEPA TPTATQAGHA LPLLPQEFPE) of human KCTD7 (NP_694578).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

ターゲット情報

画像

  • Anti-KCTD7 antibody (ab83237) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa


  • Predicted band size : 33 kDa
    Western Blot using ab83237 at 1.0ug/ml. Sample type: Hum. Adult Placenta Lysate


  • Predicted band size : 33 kDa
    Western Blot using ab83237 at 1.0ug/ml. Sample type: Hum. Fetal Heart Lysate


  • Predicted band size : 33 kDa
    Western Blot using ab83237 at 1.0ug/ml. Sample type: Hum. Fetal Lung Lysate

プロトコール

参考文献

This product has been referenced in:
  • Staropoli JF  et al. A homozygous mutation in KCTD7 links neuronal ceroid lipofuscinosis to the ubiquitin-proteasome system. Am J Hum Genet 91:202-8 (2012). Read more (PubMed: 22748208) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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